Temperature–pH sensitivity of bovine serum albumin protein-microgels based on cross-linked poly( N-isopropylacrylamide- co-acrylic acid)

Dongxia Huo; Yana Li; Qingwen Qian; Takaomi Kobayashi

doi:10.1016/j.colsurfb.2006.03.020

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Temperature–pH sensitivity of bovine serum albumin protein-microgels based on cross-linked poly( N-isopropylacrylamide- co-acrylic acid)

Journal article

Peer reviewed

Temperature–pH sensitivity of bovine serum albumin protein-microgels based on cross-linked poly( N-isopropylacrylamide- co-acrylic acid)

Dongxia Huo, Yana Li, Qingwen Qian and Takaomi Kobayashi

Colloids and surfaces, B, Biointerfaces, Vol.50(1), pp.36-42

06/01/2006

DOI: 10.1016/j.colsurfb.2006.03.020

PMID: 16698239

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Abstract

Monodispersed poly( N-isopropylacrylamide- co-acrylic acid) (PNIPAM/AAc) microgels with various contents of acrylic acid (AAc) and cross-linker N, N′-methylenebisacrylamide (MBAAm) were synthesized by surfactant-free emulsion polymerization. The microgels exhibited affinity of binding bovine serum albumin (BSA) with increase of AAc contents. The adsorption amount of BSA was strongly dependent on suspension pH. The maximum adsorption was observed at around pH 4.0, while both low pH and basic conditions induced the rather low adsorption. In addition, AFM images showed that the microgel particles underwent bridging aggregation by loading BSA. The resultant BSA-microgel particles became more hydrophobic after loading BSA. Importantly, the adsorption of BSA onto PNIPAM/AAc microgel was found to alter the swelling/deswelling behavior of the resultant microgel suspensions. With the increase of BSA concentration, the volume phase transition temperature of BSA-microgel particles shifted to the lower temperature.

Acrylic acid

Bovine serum albumin

Microgel

N-isopropylacrylamide

Volume phase transition

Details

Title: Subtitle: Temperature–pH sensitivity of bovine serum albumin protein-microgels based on cross-linked poly( N-isopropylacrylamide- co-acrylic acid)
Creators: Dongxia Huo - Nagaoka University of Technology
Yana Li - Zhengzhou University
Qingwen Qian - First Affiliated Hospital of Zhengzhou University
Takaomi Kobayashi - Nagaoka University of Technology
Resource Type: Journal article
Publication Details: Colloids and surfaces, B, Biointerfaces, Vol.50(1), pp.36-42
Publisher: Elsevier B.V
DOI: 10.1016/j.colsurfb.2006.03.020
PMID: 16698239
ISSN: 0927-7765
eISSN: 1873-4367
Language: English
Date published: 06/01/2006
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984420845602771

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