Journal article
Temporal Small RNA Expression Profiling under Drought Reveals a Potential Regulatory Role of Small Nucleolar RNAs in the Drought Responses of Maize
The plant genome, Vol.12(1), pp.1-15
03/2019
DOI: 10.3835/plantgenome2018.08.0058
PMID: 30951096
Abstract
Core Ideas
Thousands of drought‐responsive small RNAs (sRNAs) were identified.
More microRNAs and sRNAs from small nucleolar RNAs (snoRNAs) are downregulated under drought.
Most 24‐nt sRNAs are from low‐repetitive intergenic regions closer to genes.
The abundance of sRNAs from ribosome RNA increases under drought.
Ribosome RNA might be destabilized by drought through reduced snoRNA activity.
Small RNAs (sRNAs) are short noncoding RNAs that play roles in many biological processes, including drought responses in plants. However, how the expression of sRNAs dynamically changes with the gradual imposition of drought stress in plants is largely unknown. We generated time‐series sRNA sequence data from maize (Zea mays L.) seedlings under drought stress (DS) and under well‐watered (WW) conditions at the same time points. Analyses of length, functional annotation, and abundance of 736,372 nonredundant sRNAs from both DS and WW data, as well as genome copy numbers at the corresponding genomic regions, revealed distinct patterns of abundance and genome organization for different sRNA classes. The analysis identified 6646 sRNAs whose regulation was altered in response to drought stress. Among drought‐responsive sRNAs, 1325 showed transient downregulation by the seventh day, coinciding with visible symptoms of drought stress. The profiles revealed drought‐responsive microRNAs, as well as other sRNAs that originated from ribosomal RNAs (rRNAs), splicing small nuclear RNAs, and small nucleolar RNAs (snoRNA). Expression profiles of their sRNA derivers indicated that snoRNAs might play a regulatory role through regulating the stability of rRNAs and splicing small nuclear RNAs under drought condition.
Details
- Title: Subtitle
- Temporal Small RNA Expression Profiling under Drought Reveals a Potential Regulatory Role of Small Nucleolar RNAs in the Drought Responses of Maize
- Creators
- Jun Zheng - Institute of Crop Science, Chinese Academy of Agricultural SciencesErliang Zeng - Univ. of IowaYicong Du - Institute of Crop Science, Chinese Academy of Agricultural SciencesCheng He - Kansas State UnivYing Hu - Univ. of FloridaZhenzhen Jiao - Institute of Crop Science, Chinese Academy of Agricultural SciencesKai Wang - Institute of Crop Science, Chinese Academy of Agricultural SciencesWenxue Li - Institute of Crop Science, Chinese Academy of Agricultural SciencesMaria Ludens - Univ. of South DakotaJunjie Fu - Institute of Crop Science, Chinese Academy of Agricultural SciencesHaiyan Wang - Kansas State UnivFrank F White - Univ. of FloridaGuoying Wang - Institute of Crop Science, Chinese Academy of Agricultural SciencesSanzhen Liu - Kansas State Univ
- Resource Type
- Journal article
- Publication Details
- The plant genome, Vol.12(1), pp.1-15
- Publisher
- Crop Science Society of America
- DOI
- 10.3835/plantgenome2018.08.0058
- PMID
- 30951096
- ISSN
- 1940-3372
- eISSN
- 1940-3372
- Number of pages
- 15
- Grant note
- National Key Research and Development Program of China (2016YFD0101002) National Natural Science Foundation of China (31330056) Simon Foundation (246077) Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences US National Science Foundation (1238189; 1741090)
- Language
- English
- Date published
- 03/2019
- Academic Unit
- Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Biostatistics; Dental Research
- Record Identifier
- 9984070611702771
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