Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis

Jamie E Kugler; Stefan Gazdoiu; Izumi Oda-Ishii; Yale J Passamaneck; Albert J Erives; Anna Di Gregorio

doi:10.1242/jcs.066910

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Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis

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Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis

Jamie E Kugler, Stefan Gazdoiu, Izumi Oda-Ishii, Yale J Passamaneck, Albert J Erives and Anna Di Gregorio

Journal of cell science, Vol.123(Pt 14), pp.2453-2463

07/15/2010

DOI: 10.1242/jcs.066910

PMCID: PMC2894658

PMID: 20592183

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Abstract

For over a century, muscle formation in the ascidian embryo has been representative of 'mosaic' development. The molecular basis of muscle-fate predetermination has been partly elucidated with the discovery of Macho1, a maternal zinc-finger transcription factor necessary and sufficient for primary muscle development, and of its transcriptional intermediaries Tbx6b and Tbx6c. However, the molecular mechanisms by which the maternal information is decoded by cis-regulatory modules (CRMs) associated with muscle transcription factor and structural genes, and the ways by which a seamless transition from maternal to zygotic transcription is ensured, are still mostly unclear. By combining misexpression assays with CRM analyses, we have identified the mechanisms through which Ciona Macho1 (Ci-Macho1) initiates expression of Ci-Tbx6b and Ci-Tbx6c, and we have unveiled the cross-regulatory interactions between the latter transcription factors. Knowledge acquired from the analysis of the Ci-Tbx6b CRM facilitated both the identification of a related CRM in the Ci-Tbx6c locus and the characterization of two CRMs associated with the structural muscle gene fibrillar collagen 1 (CiFCol1). We use these representative examples to reconstruct how compact CRMs orchestrate the muscle developmental program from pre-localized ooplasmic determinants to differentiated larval muscle in ascidian embryos.

Ciona intestinalis - embryology

Egg Proteins - metabolism

Fibrillar Collagens - metabolism

Transcriptional Activation - genetics

Fibrillar Collagens - genetics

Transgenes - genetics

Embryonic Development - genetics

Transcription Factors - genetics

Ciona intestinalis - genetics

Regulatory Sequences, Nucleic Acid - genetics

Transcription Factors - metabolism

Ciona intestinalis - metabolism

Animals

Gene Expression Regulation, Developmental

Cloning, Molecular

Embryo, Nonmammalian

Muscle Development - genetics

Protein Binding

Egg Proteins - genetics

Details

Title: Subtitle: Temporal regulation of the muscle gene cascade by Macho1 and Tbx6 transcription factors in Ciona intestinalis
Creators: Jamie E Kugler - Department of Cell and Developmental Biology, Weill Medical College of Cornell University, 1300 York Avenue, Box 60, New York, NY 10065, USA
Stefan Gazdoiu
Izumi Oda-Ishii
Yale J Passamaneck
Albert J Erives
Anna Di Gregorio
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.123(Pt 14), pp.2453-2463
DOI: 10.1242/jcs.066910
PMID: 20592183
PMCID: PMC2894658
NLM abbreviation: J Cell Sci
ISSN: 0021-9533
eISSN: 1477-9137
Publisher: England
Grant note: R01 HD050704 / NICHD NIH HHS R01HD050704 / NICHD NIH HHS
Language: English
Date published: 07/15/2010
Academic Unit: Biology
Record Identifier: 9983991967302771

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