Testosterone (T)-induced changes in arcuate nucleus cocaine-amphetamine-regulated transcript and NPY mRNA are attenuated in old compared to young male brown Norway rats: contribution of T to age-related changes in cocaine-amphetamine-regulated transcript and NPY gene expression

Elliott H Sohn; Tami Wolden-Hanson; Alvin M Matsumoto

doi:10.1210/en.143.3.954

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Testosterone (T)-induced changes in arcuate nucleus cocaine-amphetamine-regulated transcript and NPY mRNA are attenuated in old compared to young male brown Norway rats: contribution of T to age-related changes in cocaine-amphetamine-regulated transcript and NPY gene expression

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Testosterone (T)-induced changes in arcuate nucleus cocaine-amphetamine-regulated transcript and NPY mRNA are attenuated in old compared to young male brown Norway rats: contribution of T to age-related changes in cocaine-amphetamine-regulated transcript and NPY gene expression

Elliott H Sohn, Tami Wolden-Hanson and Alvin M Matsumoto

Endocrinology (Philadelphia), Vol.143(3), pp.954-963

03/2002

DOI: 10.1210/en.143.3.954

PMID: 11861518

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Abstract

The age-related decrease in serum T levels is associated with impairments in food intake and weight regulation and alterations in brain peptides that regulate energy balance. To test the hypothesis that reduced T levels contribute to altered hypothalamic cocaine-amphetamine-regulated transcript (CART) and NPY gene expression, the mRNA content of these neuropeptides was measured by in situ hybridization in sham-operated (intact), castrated, and T-replaced castrated young and old male Brown Norway rats. T levels in T-replaced young and old rats were similar to those in intact young animals. Compared with castrated rats, arcuate nucleus CART mRNA was lower and NPY mRNA was higher in both young and old T-replaced castrated animals, suggesting reciprocal regulation of these peptides by T; these T-induced changes were localized primarily in the rostral arcuate and were markedly attenuated in old animals. Compared with intact animals, paraventricular nucleus CART mRNA was lower in castrated animals and similar in T-replaced young and old rats. We conclude that hypothalamic CART and NPY neurons remain responsive to T regulation in old rats, albeit less so than in young animals, suggesting that the age-related reduction of T contributes in part to altered brain neuropeptide gene expression favoring anorexia and wasting with aging.

Transcription, Genetic - drug effects

Dopamine Uptake Inhibitors - pharmacology

Arcuate Nucleus of Hypothalamus - metabolism

Amphetamine - pharmacology

Body Weight - drug effects

Rats

Male

Neuropeptide Y - biosynthesis

Testosterone - pharmacology

Nerve Tissue Proteins - genetics

Orchiectomy

Cocaine - pharmacology

RNA, Messenger - biosynthesis

Rats, Inbred BN

Animals

Hypothalamus - metabolism

In Situ Hybridization

Body Composition - drug effects

Arcuate Nucleus of Hypothalamus - drug effects

Nerve Tissue Proteins - biosynthesis

Hypothalamus - drug effects

Aging - metabolism

Details

Title: Subtitle: Testosterone (T)-induced changes in arcuate nucleus cocaine-amphetamine-regulated transcript and NPY mRNA are attenuated in old compared to young male brown Norway rats: contribution of T to age-related changes in cocaine-amphetamine-regulated transcript and NPY gene expression
Creators: Elliott H Sohn - Geriatric Research, Education, and Clinical Center, Veterans Administration Puget Sound Health Care System, Seattle, Washington 98108-1597, USA
Tami Wolden-Hanson
Alvin M Matsumoto
Resource Type: Journal article
Publication Details: Endocrinology (Philadelphia), Vol.143(3), pp.954-963
Publisher: United States
DOI: 10.1210/en.143.3.954
PMID: 11861518
ISSN: 0013-7227
eISSN: 1945-7170
Grant note: P50-HD-12629 / NICHD NIH HHS
Language: English
Date published: 03/2002
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9983980292002771

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