Journal article
Testosterone receptor blockade restores cellular immunity in male mice after burn injury
Journal of endocrinology, Vol.169(2), pp.299-308
2001
DOI: 10.1677/joe.0.1690299
PMID: 11312147
Abstract
Males are known to have increased risk for septic complications after traumatic injury, which appears to be mediated by the inhibitory effects of testosterone on immune function. The role of testosterone in immunity after burn injury, however, remains unclear. Herein, we examined the effects of a testosterone receptor antagonist, flutamide, on delayed type hypersensitivity response (DTH), splenocyte proliferation, interleukin (IL)-2 secretion, and IL-2 receptor (IL-2R) expression in male BALB/c mice subjected to a 15% total body surface area burn or sham injury. Burn- or sham-injured mice were given flutamide s.c. at 30 min and 24 h after injury. At 48 h, burn injury caused a 48% (P<0.001) decrease in DTH response; however, mice that received flutamide treatment did not demonstrate significant suppression of DTH. Likewise, splenocyte proliferation and IL-2 production were depressed in burned animals in comparison with sham-injured controls, and flutamide treatment resulted in a partial restoration of these responses. In vitro studies indicated that splenocytes from sham- and burn-injured mice were equally sensitive to the suppressive effects of 5alpha-dihydrotestosterone in regard to proliferation and IL-2 production. Further evaluation revealed a decrease in IL-2R expression on splenocytes from burned mice and a partial restoration of this expression with flutamide treatment. Thus blocking testosterone receptor activation improves the cellular immunity in thermally injured mice, possibly through restoration of IL-2 production and IL-2R expression. It remains to be determined whether the effects of testosterone in this injury model are direct or indirect.
Details
- Title: Subtitle
- Testosterone receptor blockade restores cellular immunity in male mice after burn injury
- Creators
- K. A. N MESSINGHAM - Department of Cell Biology, Neurobiology and Anatomy, Loyola University Medical Center, Maywood, Illinois 60153, United StatesM SHIRAZI - Stritch School of Medicine, Loyola University Medical Center, Maywood, Illinois 60153, United StatesL. A DUFFNER - The Burn and Shock Trauma Institute, Loyola University Medical Center, Maywood, Illinois 60153, United StatesM. A EMANUELE - Department of Medicine, Loyola University Medical Center, Maywood, Illinois 60153, United StatesE. J KOVACS - Department of Cell Biology, Neurobiology and Anatomy, Loyola University Medical Center, Maywood, Illinois 60153, United States
- Resource Type
- Journal article
- Publication Details
- Journal of endocrinology, Vol.169(2), pp.299-308
- Publisher
- Portland Press; Colchester
- DOI
- 10.1677/joe.0.1690299
- PMID
- 11312147
- ISSN
- 0022-0795
- eISSN
- 1479-6805
- Language
- English
- Date published
- 2001
- Academic Unit
- Dermatology
- Record Identifier
- 9984025321202771
Metrics
17 Record Views