Journal article
Th17-Inducing Conditions Lead to in vitro Activation of Both Th17 and Th1 Responses in Behcet's Disease
Immunological investigations, Vol.46(5), pp.518-525
07/04/2017
DOI: 10.1080/08820139.2017.1306865
PMID: 28414558
Abstract
Objectives: Interleukin-17 (IL-17) has been associated with the pathogenesis of various autoimmune/inflammatory diseases. The aim of this study was to investigate the expression of Th17-related immunity in an innate immunity-dominated vasculitis, namely Behcet's disease (BD).
Methods: Peripheral blood mononuclear cells from 37 patients (age: 38.5 ± 9.8 years) with BD, and 25 healthy controls (HC) (age: 39.1 ± 9.3 years), were cultured in Th17-inducing conditions (IL-6, Phytohemagglutinin (PHA), IL-1β, and IL-23) for 6 days. Cultured cells were stained with CD4, CD8, CD3, TCR gamma/delta, CD19, interferon-γ (IFN-γ), and IL-17 antibodies to determine the intracellular cytokine secretion by flow cytometry.
Results: IL-17 expression by CD8+ and γδ+ T cells was higher in BD compared to HC (p = 0.004, p = 0.003, respectively). No differences were observed between the groups in the IL-17 production by B cells. Under Th17-inducing conditions, production of IFN-γ by CD4+, CD8+, and γδ+ T cells was also higher in BD compared to HC (p < 0.05 in all).
Conclusion: Our results suggest that under Th17-stimulating conditions, T cells express both IL-17 and IFN-γ in BD. More prominent IL-17 and IFN-γ production by all lymphocyte subsets in BD might be associated with the increased innate responses, early tissue neutrophil infiltrations and late adaptive immunity in BD.
Details
- Title: Subtitle
- Th17-Inducing Conditions Lead to in vitro Activation of Both Th17 and Th1 Responses in Behcet's Disease
- Creators
- Rabia Deniz - Marmara UniversityAysın Tulunay-Virlan - Marmara UniversityFiliz Ture Ozdemir - Marmara UniversityAli Ugur Unal - Marmara UniversityGulsen Ozen - Marmara UniversityFatma Alibaz-Oner - Marmara UniversityImren Aydin-Tatli - Marmara UniversityGonca Mumcu - Marmara UniversityTulin Ergun - Marmara UniversityHaner Direskeneli - Marmara University
- Resource Type
- Journal article
- Publication Details
- Immunological investigations, Vol.46(5), pp.518-525
- Publisher
- Taylor & Francis
- DOI
- 10.1080/08820139.2017.1306865
- PMID
- 28414558
- ISSN
- 0882-0139
- eISSN
- 1532-4311
- Number of pages
- 8
- Grant note
- Project No: SAG-A-090512.0132 / Marmara University Research Fund (BAPKO)
- Language
- English
- Date published
- 07/04/2017
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984702900002771
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