Journal article
The -2518bp promoter polymorphism at CCL2/MCP1 influences susceptibility to mucosal but not localized cutaneous leishmaniasis in Brazil
Infection, genetics and evolution, Vol.10(5), pp.607-613
07/2010
DOI: 10.1016/j.meegid.2010.04.006
PMCID: PMC2878927
PMID: 20430117
Abstract
Mucosal leishmaniasis (ML) follows localized cutaneous leishmaniasis (CL) caused by Leishmania braziliensis. Proinflammatory responses mediate CL self-healing but are exaggerated in ML. Proinflammatory monocyte chemoattractant protein 1 (MCP-1; encoded by CCL2) is associated with CL. We explore its role in CL/ML through analysis of the regulatory CCL2 -2518bp promoter polymorphism in CL/ML population samples and families from Brazil. Genotype frequencies were compared among ML/CL cases and control groups using logistic regression and the family-based association test (FBAT). MCP-1 was measured in plasma and macrophages. The GG recessive genotype at CCL2 -2518bp was more common in patients with ML (N=67) than in neighborhood control (NC; N=60) subjects (OR 1.78; 95% CI 1.01-3.14; P=0.045), than in NC combined with leishmanin skin-test positive (N=60) controls (OR 4.40; 95% CI 1.42-13.65; P=0.010), and than in controls combined with CL (N=60) patients (OR 2.78; 95% CI 1.13-6.85; P=0.045). No associations were observed for CL compared to any groups. FBAT (91 ML and 223 CL cases in families) confirmed recessive association of ML with allele G (Z=2.679; P=0.007). Higher levels of MCP-1 occurred in plasma (P=0.03) and macrophages (P<0.0001) from GG compared to AA individuals. These results suggest that high MCP-1 increases risk of ML.
Details
- Title: Subtitle
- The -2518bp promoter polymorphism at CCL2/MCP1 influences susceptibility to mucosal but not localized cutaneous leishmaniasis in Brazil
- Creators
- Rajendranath Ramasawmy - Universidade Federal da Bahia, Salvador, BrazilEliane MenezesAndrea MagalhãesJoyce OliveiraLéa CastellucciRoque AlmeidaMaria Elisa A RosaLuiz Henrique GuimarãesMarcus LessaElza NoronhaMary E WilsonSarra E JamiesonJorge KalilJenefer M BlackwellEdgar M CarvalhoAmélia Ribeiro de Jesus
- Resource Type
- Journal article
- Publication Details
- Infection, genetics and evolution, Vol.10(5), pp.607-613
- DOI
- 10.1016/j.meegid.2010.04.006
- PMID
- 20430117
- PMCID
- PMC2878927
- NLM abbreviation
- Infect Genet Evol
- ISSN
- 1567-1348
- eISSN
- 1567-7257
- Publisher
- Netherlands
- Grant note
- P50 AI030639-18 / NIAID NIH HHS Wellcome Trust R01AI067874 / NIAID NIH HHS R01 AI067874-05 / NIAID NIH HHS R01 AI076233-01A1 / NIAID NIH HHS R03 TW001369 / FIC NIH HHS R01 AI048822 / NIAID NIH HHS R03AI070909 / NIAID NIH HHS P50 AI-30639 / NIAID NIH HHS R03 AI070909 / NIAID NIH HHS 1 D43 TW007127-01 / FIC NIH HHS D43 TW007127 / FIC NIH HHS R01 AI067874 / NIAID NIH HHS R01 AI048822-05 / NIAID NIH HHS R01 AI076233 / NIAID NIH HHS R03 TW001369-03 / FIC NIH HHS P50 AI030639 / NIAID NIH HHS
- Language
- English
- Date published
- 07/2010
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Internal Medicine
- Record Identifier
- 9984001132902771
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