Journal article
The Ability of Positive Transcription Elongation Factor b To Transactivate Human Immunodeficiency Virus Transcription Depends on a Functional Kinase Domain, Cyclin T1, and Tat
Journal of virology, Vol.72(9), pp.7154-7159
09/1998
DOI: 10.1128/JVI.72.9.7154-7159.1998
PMCID: PMC109937
PMID: 9696809
Abstract
By binding to the transactivation response element (TAR) RNA, the transcriptional transactivator (Tat) from the human immunodeficiency virus increases rates of elongation rather than initiation of viral transcription. Two cyclin-dependent serine/threonine kinases, CDK7 and CDK9, which phosphorylate the C-terminal domain of RNA polymerase II, have been implicated in Tat transactivation in vivo and in vitro. In this report, we demonstrate that CDK9, which is the kinase component of the positive transcription elongation factor b (P-TEFb) complex, can activate viral transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion proteins (Rev). The kinase activity of CDK9 and cyclin T1 is essential for these effects. Moreover, P-TEFb binds to TAR only in the presence of Tat. We conclude that Tat–P-TEFb complexes bind to TAR, where CDK9 modifies RNA polymerase II for the efficient copying of the viral genome.
Details
- Title: Subtitle
- The Ability of Positive Transcription Elongation Factor b To Transactivate Human Immunodeficiency Virus Transcription Depends on a Functional Kinase Domain, Cyclin T1, and Tat
- Creators
- Koh Fujinaga - Departments of Medicine, Microbiology, and Immunology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143-0703Thomas P Cujec - Departments of Medicine, Microbiology, and Immunology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143-0703Junmin Peng - Departments of Medicine, Microbiology, and Immunology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143-0703Judit Garriga - Departments of Medicine, Microbiology, and Immunology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143-0703David H Price - Departments of Medicine, Microbiology, and Immunology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143-0703Xavier Graña - Departments of Medicine, Microbiology, and Immunology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143-0703B. Matija Peterlin - Departments of Medicine, Microbiology, and Immunology, Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California 94143-0703
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.72(9), pp.7154-7159
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/JVI.72.9.7154-7159.1998
- PMID
- 9696809
- PMCID
- PMC109937
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 09/1998
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984024404602771
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