The Bacteriology of Gangrenous and Perforated Appendicitis—Revisited

ROBERT S. Bennion; ELLEN J. Baron; JESSE E. Thompson; JULIA Downes; PAULA Summanen; DAVID A. Talan; SYDNEY M. Finegold

doi:10.1097/00000658-199002000-00008

Back

The Bacteriology of Gangrenous and Perforated Appendicitis—Revisited

Journal article

Open access

Peer reviewed

The Bacteriology of Gangrenous and Perforated Appendicitis—Revisited

ROBERT S. Bennion, ELLEN J. Baron, JESSE E. Thompson, JULIA Downes, PAULA Summanen, DAVID A. Talan and SYDNEY M. Finegold

Annals of surgery, Vol.211(2), pp.165-171

02/1990

DOI: 10.1097/00000658-199002000-00008

PMCID: PMC1357960

PMID: 2405791

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1357960View

Published (Version of record) Open Access

Abstract

By using optimum sampling, transport, and culture techniques in patients with gangrenous or perforated appendicitis, we recovered larger numbers of bacteria with a more diverse flora than has previously been reported. Thirty patients older than 12 years with histologically documented gangrenous or perforated appendicitis had peritoneal fluid, appendiceal tissue, and abscess contents (if present) cultured. Appendiceal tissue was obtained so as to exclude the lumen. A total of 223 anaerobes and 82 aerobic or faculatative bacteria were recovered, an average of 10.2 different organisms per specimen. Twenty-one different genera and more than 40 species were encountered. Bacteroides fragilis group and Escherichia coli were isolated from almost all specimens. Within the B. fragilis group, eight species were represented. Other frequent isolates included Peptostreptococcus (80%), Pseudomonas (40% [P. aeruginosa, 23.3%, other Pseudomonas spp., 16.7%]), B. splanchnicus (40%), B. intermedius (36.7%), and Lactobacillus (36.7%). Interestingly a previously undescribed fastidious gram-negative anaerobic bacillus was isolated from nearly one half of all patients. This organism was found to have low DNA homology (by dot blot) with the known organisms most closely resembling it.

Details

Title: Subtitle: The Bacteriology of Gangrenous and Perforated Appendicitis—Revisited
Creators: ROBERT S. Bennion - Department of Surgery, Olive View Medical Center, Los Angeles, California.
ELLEN J. Baron
JESSE E. Thompson
JULIA Downes
PAULA Summanen
DAVID A. Talan
SYDNEY M. Finegold
Resource Type: Journal article
Publication Details: Annals of surgery, Vol.211(2), pp.165-171
DOI: 10.1097/00000658-199002000-00008
PMID: 2405791
PMCID: PMC1357960
ISSN: 0003-4932
eISSN: 1528-1140
Language: English
Date published: 02/1990
Academic Unit: Emergency Medicine; Internal Medicine
Record Identifier: 9984296984502771

Metrics

28 Record Views

147 Times Cited - Web of Science