Journal article
The CD45RB-Associated Epitope Defined by Monoclonal Antibody CZ-1 Is an Activation and Memory Marker for Mouse CD4 T Cells
Cellular immunology, Vol.167(1), pp.56-62
01/10/1996
DOI: 10.1006/cimm.1996.0007
PMID: 8548845
Abstract
Monoclonal antibody CZ-1 defines a novel sialic acid-dependent CD45RB-associated epitope. The CZ-1 antigen is expressed on the subpopulation of CD4 T cells that proliferate in response to IL-2. Because IL-2 responsiveness often denotes T cell activation, we examined the expression of the CZ-1 antigen on CD4 T cells taken from mice at various times during an infection with lymphocytic choriomeningitis virus (LCMV). The blast-sized CD4 T cells at Day 6 postinfection were CZ-1+. Further cell surface phenotyping showed that those blast cells activated at Day 6 postinfection were CD45RBdimPgp-1brightMEL-14−. This contrasts with the CZ-1−CD45RBbrightPgp-1bright/dimMEL-14+resting cell population prior to infection. To determine if memory CD4 T cells continued to express the CZ-1 epitope long after resolution of the LCMV infection, CD4+CZ-1+and CD4+CZ-1−populations were purified by cell sorting and placed in anin vitroproliferation assay with LCMV-infected antigen-presenting cells. It was found that the CD4+CZ-1+population contained virtually all of the virus-specific memory. The CZ-1 epitope is therefore both an activation and a memory marker for murine CD4 T cells.
Details
- Title: Subtitle
- The CD45RB-Associated Epitope Defined by Monoclonal Antibody CZ-1 Is an Activation and Memory Marker for Mouse CD4 T Cells
- Creators
- Steven M VargaRaymond M Welsh
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.167(1), pp.56-62
- Publisher
- Elsevier Inc
- DOI
- 10.1006/cimm.1996.0007
- PMID
- 8548845
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 01/10/1996
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology
- Record Identifier
- 9984083879702771
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