The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle

Annelise O Jorgensen; Amy C.-Y Shen; Wayne Arnold; Peter S Mcpherson; Kevin P Campbell

doi:10.1083/jcb.120.4.969

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The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle

Journal article

Open access

Peer reviewed

The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle

Annelise O Jorgensen, Amy C.-Y Shen, Wayne Arnold, Peter S Mcpherson and Kevin P Campbell

The Journal of cell biology, Vol.120(4), pp.969-980

02/1993

DOI: 10.1083/jcb.120.4.969

PMCID: PMC2200068

PMID: 8381786

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Abstract

The subcellular distribution of the Ca(2+)-release channel/ryanodine receptor in adult rat papillary myofibers has been determined by immunofluorescence and immunoelectron microscopical studies using affinity purified antibodies against the ryanodine receptor. The receptor is confined to the sarcoplasmic reticulum (SR) where it is localized to interior and peripheral junctional SR and the corbular SR, but it is absent from the network SR where the SR-Ca(2+)-ATPase and phospholamban are densely distributed. Immunofluorescence labeling of sheep Purkinje fibers show that the ryanodine receptor is confined to discrete foci while the SR-Ca(2+)-ATPase is distributed in a continuous network-like structure present at the periphery as well as throughout interior regions of these myofibers. Because Purkinje fibers lack T-tubules, these results indicate that the ryanodine receptor is localized not only to the peripheral junctional SR but also to corbular SR densely distributed in interfibrillar spaces of the I-band regions. We have previously identified both corbular SR and junctional SR in cardiac muscle as potential Ca(2+)-storage/Ca(2+)-release sites by demonstrating that the Ca2+ binding protein calsequestrin and calcium are very densely distributed in these two specialized domains of cardiac SR in situ. The results presented here provide strong evidence in support of the hypothesis that corbular SR is indeed a site of Ca(2+)-induced Ca2+ release via the ryanodine receptor during excitation contraction coupling in cardiac muscle. Furthermore, these results indicate that the function of the cardiac Ca(2+)-release channel/ryanodine receptor is not confined to junctional complexes between SR and the sarcolemma.

Immunohistochemistry

Antibody Specificity

Purkinje Cells - metabolism

Calcium Channels - metabolism

Receptors, Cholinergic - metabolism

Calcium - metabolism

Ryanodine Receptor Calcium Release Channel

Rats

Animals

Muscle Contraction

Myocardium - metabolism

Fluorescent Antibody Technique

Receptors, Cholinergic - immunology

Sheep

Sarcoplasmic Reticulum - metabolism

Sarcoplasmic Reticulum - immunology

Myocardium - ultrastructure

Details

Title: Subtitle: The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle
Creators: Annelise O Jorgensen - Department of Anatomy and Cell Biology, University of Toronto, Ontario, Canada
Amy C.-Y Shen
Wayne Arnold
Peter S Mcpherson
Kevin P Campbell
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.120(4), pp.969-980
Publisher: United States
DOI: 10.1083/jcb.120.4.969
PMID: 8381786
PMCID: PMC2200068
ISSN: 0021-9525
eISSN: 1540-8140
Grant note: HL39265 / NHLBI NIH HHS
Language: English
Date published: 02/1993
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984068273402771

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