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The Candida albicans transcription factor Efg1 governs hyphal morphogenesis independently of the cAMP-protein kinase A pathway
Journal article   Open access   Peer reviewed

The Candida albicans transcription factor Efg1 governs hyphal morphogenesis independently of the cAMP-protein kinase A pathway

Juraj Kramara, Rohan S. Wakade, Corey Frazer, Mark A. Stamnes, Richard J. Bennett and Damian J. Krysan
mBio, Vol.16(12), e0291325
12/10/2025
DOI: 10.1128/mbio.02913-25
PMCID: PMC12691644
PMID: 41170998
url
https://doi.org/10.1128/mbio.02913-25View
Published (Version of record) Open Access

Abstract

Candida albicans is one of the most common causes of human fungal disease. An intensively studied C. albicans virulence traits is its ability to adopt both yeast and filamentous morphologies. Accordingly, the regulation of the yeast-to-filament transition has been an area of intense study in medical mycology. A long-standing mechanistic paradigm in this field is that the cAMP-protein kinase A pathway phosphorylates a master transcriptional regulator of C. albicans filamentation, Efg1, to drive filamentation. This model has been generalized over the years despite the fact that the initial work showed that it applied to only specific in vitro filamentation conditions. Here, we reinvestigated the protein kinase A-Efg1 paradigm by generating new strains containing alleles of EFG1 with the putative protein kinase A phosphorylation site (T208) mutated to either Ala or Glu which blocks or mimics phosphorylation, respectively. We integrated these alleles into efg1∆∆ mutants so that they are expressed from the endogenous promoter. We assayed the effect of these Efg1 mutations on (i) in vitro filamentation under a wide range of inducing conditions, (ii) biofilm formation, (iii) in vivo filamentation, (iv) virulence in a model of disseminated candidiasis, and (v) gene expression during in vitro filamentation. Using updated genetic approaches, we found no evidence that blocking or mimicking protein kinase phosphorylation of Efg1 affected its function during filamentation, biofilm formation, or infection. Therefore, additional studies will be required to identify the mechanisms by which Efg1 is regulated during hyphal morphogenesis.IMPORTANCECandida albicans is a common human fungal pathogen causing both superficial mucosal and life-threatening invasive disease. The virulence of C. albicans is associated with its ability to form filamentous hyphae and pseudohyphae. The regulation of this process is widely attributed to the phosphorylation of a transcription factor Efg1 by the protein kinase A signaling pathway. Since its initial description 25 years ago, this model has informed the design and interpretation of many studies of C. albicans. Through the use of updated genetic methods, we have found that protein kinase A-mediated phosphorylation of Efg1 is dispensable for filamentation, biofilm formation, and virulence in an experimental model of candidiasis. Although these data are negative in nature, the centrality of the protein kinase A-Efg1 paradigm to C. albicans pathogenesis studies increases their impact and should catalyze new studies to understand how this master regulator of C. albicans biology is itself regulated.
 Mycology Candida Albicans Pathogenesis Dimorphic Yeast Eukaryotic Microbiology Fungal Morphogenesis Fungal Morphology Fungal Pathogenesis Microbial Pathogenesis and Immunology Research Article Yeast and Filamentous Forms Yeast Biology Yeast Hyphal Transition Yeast Pathogenicity and Host Interactions

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