The Chx10-Traf3 Knockout Mouse as a Viable Model to Study Neuronal Immune Regulation

Jami M. Gurley; Grzegorz B. Gmyrek; Elizabeth A. Hargis; Gail A. Bishop; Daniel J. J. Carr; Michael H. Elliott

doi:10.3390/cells10082068

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The Chx10-Traf3 Knockout Mouse as a Viable Model to Study Neuronal Immune Regulation

Journal article

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The Chx10-Traf3 Knockout Mouse as a Viable Model to Study Neuronal Immune Regulation

Jami M. Gurley, Grzegorz B. Gmyrek, Elizabeth A. Hargis, Gail A. Bishop, Daniel J. J. Carr and Michael H. Elliott

Cells (Basel, Switzerland), Vol.10(8), p.2068

08/01/2021

DOI: 10.3390/cells10082068

PMCID: PMC8391412

PMID: 34440839

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Abstract

Uncontrolled inflammation is associated with neurodegenerative conditions in central nervous system tissues, including the retina and brain. We previously found that the neural retina (NR) plays an important role in retinal immunity. Tumor necrosis factor Receptor-Associated Factor 3 (TRAF3) is a known immune regulator expressed in the retina; however, whether TRAF3 regulates retinal immunity is unknown. We have generated the first conditional NR-Traf3 knockout mouse model (Chx10-Cre/Traf3(f/f)) to enable studies of neuronal TRAF3 function. Here, we evaluated NR-Traf3 depletion effects on whole retinal TRAF3 protein expression, visual acuity, and retinal structure and function. Additionally, to determine if NR-Traf3 plays a role in retinal immune regulation, we used flow cytometry to assess immune cell infiltration following acute local lipopolysaccharide (LPS) administration. Our results show that TRAF3 protein is highly expressed in the NR and establish that NR-Traf3 depletion does not affect basal retinal structure or function. Importantly, NR-Traf3 promoted LPS-stimulated retinal immune infiltration. Thus, our findings propose NR-Traf3 as a positive regulator of retinal immunity. Further, the NR-Traf3 mouse provides a tool for investigations of neuronal TRAF3 as a novel potential target for therapeutic interventions aimed at suppressing retinal inflammatory disease and may also inform treatment approaches for inflammatory neurodegenerative brain conditions.

Cell Biology

Life Sciences & Biomedicine

Science & Technology

Details

Title: Subtitle: The Chx10-Traf3 Knockout Mouse as a Viable Model to Study Neuronal Immune Regulation
Creators: Jami M. Gurley - University of Oklahoma Health Sciences Center
Grzegorz B. Gmyrek - University of Oklahoma Health Sciences Center
Elizabeth A. Hargis - University of Oklahoma Health Sciences Center
Gail A. Bishop - University of Iowa
Daniel J. J. Carr - University of Oklahoma Health Sciences Center
Michael H. Elliott - University of Oklahoma Health Sciences Center
Resource Type: Journal article
Publication Details: Cells (Basel, Switzerland), Vol.10(8), p.2068
DOI: 10.3390/cells10082068
PMID: 34440839
PMCID: PMC8391412
NLM abbreviation: Cells
ISSN: 2073-4409
eISSN: 2073-4409
Publisher: Mdpi
Number of pages: 17
Grant note: HF18-00 / Postdoctoral OCAST Fellowship grant Research to Prevent Blindness; Research to Prevent Blindness (RPB)
Language: English
Date published: 08/01/2021
Academic Unit: Microbiology and Immunology; President; Fraternal Order of Eagles Diabetes Research Center
Record Identifier: 9984297429002771

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