Journal article
The Drosophila nuclear lamina protein Otefin is required for germline stem cell survival
Developmental cell, Vol.25(6), pp.645-654
06/24/2013
DOI: 10.1016/j.devcel.2013.05.023
PMCID: PMC3710436
PMID: 23806619
Abstract
LEM domain (LEM-D) proteins are components of an extensive protein network that assembles beneath the inner nuclear envelope. Defects in LEM-D proteins cause tissue-restricted human diseases associated with altered stem cell homeostasis. Otefin (Ote) is a Drosophila LEM-D protein that is intrinsically required for female germline stem cells (GSCs) maintenance. Previous studies linked Ote loss with transcriptional activation of the key differentiation gene,
bag-of-marbles
(
bam
), leading to the model that Ote tethers the
bam
gene to the nuclear periphery for gene silencing. Using genetic and phenotypic analyses of multiple
ote
−/−
backgrounds, we obtained evidence that is inconsistent with this model. We show that
bam
repression is maintained in
ote
−/−
GSCs and that germ cell loss persists in
ote
−/−
, bam
−/−
mutants, together demonstrating that GSC loss is independent of
bam
transcription. We show the primary defect in
ote
−/−
GSCs is a block of differentiation, which ultimately leads to germ cell death.
Details
- Title: Subtitle
- The Drosophila nuclear lamina protein Otefin is required for germline stem cell survival
- Creators
- Lacy J Barton - Department of Biochemistry, University of Iowa, College of Medicine, Iowa City, IA-52242, USA., Phone #: 319-335-6953; FAX # 319-384-4770Belinda S Pinto - Molecular and Cellular Biology Program, University of Iowa, College of Medicine, Iowa City, IA-52242, USA., Phone #: 319-335-6953; FAX # 319-384-4770Lori L Wallrath - Department of Biochemistry, University of Iowa, College of Medicine, Iowa City, IA-52242, USA., Phone #: 319-335-6953; FAX # 319-384-4770Pamela K Geyer - Department of Biochemistry, University of Iowa, College of Medicine, Iowa City, IA-52242, USA., Phone #: 319-335-6953; FAX # 319-384-4770
- Resource Type
- Journal article
- Publication Details
- Developmental cell, Vol.25(6), pp.645-654
- DOI
- 10.1016/j.devcel.2013.05.023
- PMID
- 23806619
- PMCID
- PMC3710436
- ISSN
- 1534-5807
- eISSN
- 1878-1551
- Grant note
- R01 GM087341 || GM / National Institute of General Medical Sciences : NIGMS
- Language
- English
- Date published
- 06/24/2013
- Academic Unit
- Obstetrics and Gynecology; Biochemistry and Molecular Biology; University College Courses
- Record Identifier
- 9984025248202771
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