Journal article
The Dynamics of Dendritic Structure in Developing Hippocampal Slices
The Journal of neuroscience, Vol.16(9), pp.2983-2994
05/01/1996
DOI: 10.1523/JNEUROSCI.16-09-02983.1996
PMCID: PMC6579052
PMID: 8622128
Abstract
Time-lapse fluorescence confocal microscopy was used to directly visualize the formation and dynamics of postsynaptic target structures (i.e., dendritic branches and spines) on pyramidal neurons within developing hippocampal tissue slices. Within a 2 week period of time, pyramidal neurons in cultured slices derived from early postnatal rat (postnatal days 2–7) developed complex dendritic arbors bearing numerous postsynaptic spines. At early stages (1–2 d
in vitro
), many fine filopodial protrusions on dendrite shafts rapidly extended (maximum rate ∼2.5 μm/min) and retracted (median filopodial lifetime, 10 min), but some filopodia transformed into growth cones and nascent dendrite branches. As dendritic arbors matured, the population of fleeting lateral filopodia was replaced by spine-like structures having a low rate of turnover. This developmental progression involved a transitional stage in which dendrites were dominated by persistent (up to 22 hr) but dynamic spiny protrusions (i.e., protospines) that showed substantial changes in length and shape on a timescale of minutes. These observations reveal a highly dynamic state of postsynaptic target structures that may actively contribute to the formation and plasticity of synaptic connections during CNS development.
Details
- Title: Subtitle
- The Dynamics of Dendritic Structure in Developing Hippocampal Slices
- Creators
- Michael E DaileyStephen J Smith
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.16(9), pp.2983-2994
- Publisher
- Society for Neuroscience
- DOI
- 10.1523/JNEUROSCI.16-09-02983.1996
- PMID
- 8622128
- PMCID
- PMC6579052
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Language
- English
- Date published
- 05/01/1996
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070485502771
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