The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function

Kate Koles; Emily M Messelaar; Zachary Feiger; Crystal J Yu; C Andrew Frank; Avital A Rodal

doi:10.1091/mbc.E15-02-0093

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The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function

Journal article

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Peer reviewed

The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function

Kate Koles, Emily M Messelaar, Zachary Feiger, Crystal J Yu, C Andrew Frank and Avital A Rodal

Molecular biology of the cell, Vol.26(18), pp.3275-3288

09/15/2015

DOI: 10.1091/mbc.E15-02-0093

PMCID: PMC4569317

PMID: 26202464

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Abstract

Membranes form elaborate structures that are highly tailored to their specialized cellular functions, yet the mechanisms by which these structures are shaped remain poorly understood. Here, we show that the conserved membrane-remodeling C-terminal Eps15 Homology Domain (EHD) protein Past1 is required for the normal assembly of the subsynaptic muscle membrane reticulum (SSR) at the Drosophila melanogaster larval neuromuscular junction (NMJ). past1 mutants exhibit altered NMJ morphology, decreased synaptic transmission, reduced glutamate receptor levels, and a deficit in synaptic homeostasis. The membrane-remodeling proteins Amphiphysin and Syndapin colocalize with Past1 in distinct SSR subdomains and collapse into Amphiphysin-dependent membrane nodules in the SSR of past1 mutants. Our results suggest a mechanism by which the coordinated actions of multiple lipid-binding proteins lead to the elaboration of increasing layers of the SSR and uncover new roles for an EHD protein at synapses.

Nerve Tissue Proteins - metabolism

Receptors, Glutamate - metabolism

Animals

Carrier Proteins - metabolism

Larva

Neuromuscular Junction - metabolism

Presynaptic Terminals - metabolism

Adaptor Proteins, Signal Transducing - metabolism

Drosophila Proteins - metabolism

Drosophila melanogaster

Synaptic Membranes - metabolism

Details

Title: Subtitle: The EHD protein Past1 controls postsynaptic membrane elaboration and synaptic function
Creators: Kate Koles - Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453
Emily M Messelaar - Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453
Zachary Feiger - Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453
Crystal J Yu - Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453
C Andrew Frank - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA 52242
Avital A Rodal - Rosenstiel Basic Medical Sciences Research Center, Department of Biology, Brandeis University, Waltham, MA 02453 arodal@brandeis.edu
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.26(18), pp.3275-3288
DOI: 10.1091/mbc.E15-02-0093
PMID: 26202464
PMCID: PMC4569317
NLM abbreviation: Mol Biol Cell
ISSN: 1059-1524
eISSN: 1939-4586
Publisher: American Society for Cell Biology; United States
Grant note: R00 NS062738 / NINDS NIH HHS K99 NS062738 / NINDS NIH HHS P40 OD018537 / NIH HHS DP2 NS082127 / NCCDPHP CDC HHS NS062738 / NINDS NIH HHS P40OD018537 / NIH HHS DP2 NS082127 / NINDS NIH HHS
Language: English
Date published: 09/15/2015
Academic Unit: Anatomy and Cell Biology; Iowa Neuroscience Institute
Record Identifier: 9984025420402771

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