Journal article
The Effect of Hyperthermia on Mitomycin-C Induced Cytotoxicity in Four Human Bladder Cancer Cell Lines
European urology, Vol.46(5), pp.670-674
2004
DOI: 10.1016/j.eururo.2004.06.009
PMID: 15474281
Abstract
Introduction:
Hyperthermia and mitomycin-C (MMC) have given very encouraging results in several clinical studies for the treatment of superficial transitional cell carcinoma of the bladder. However, a synergistic effect of hyperthermia and MMC on the decrease of cell proliferation has never been demonstrated accurately
in vitro. We investigated the effect of MMC versus MMC combined with hyperthermia on the cytotoxicity in four human bladder cancer cell lines.
Material and Methods:
The RT112, RT4, 253J and T24 human bladder cancer cell lines were seeded in 96-well microtiter plates at 2.0 × 10
4 cells per well and were left to attach for 24
hours. The cells were then treated for 60
minutes with MMC concentrations ranging from 0 to 400
μg/ml at a temperature of 37
°C or 43
°C. After treatment cells were rinsed three times with culture medium and left for 24
hours in the incubator. Dimethyl thiazolyl tetrazolium (MTT) solution was added and after 4
hours of incubation the MTT containing media was aspired from all wells and 100
μl of dimethyl sulfoxide was added to each well. A spectrum analyses was performed at 595
nm light wavelength.
Results:
A decrease of cell proliferation after treatment with increasing concentrations MMC was demonstrated. Hyperthermia has a synergistic effect on the decrease of cell proliferation by different concentrations MMC. In the cells treated without MMC no significant difference in the extent of cell killing at 37
°C and 43
°C was observed. Furthermore, no difference was observed between cells with a p53 protein mutation (RT112 and T24) or without a p53 protein mutation (253J and RT4).
Conclusion:
A clear synergistic effect of MMC and hyperthermia has been demonstrated in four human bladder cancer cell lines.
Details
- Title: Subtitle
- The Effect of Hyperthermia on Mitomycin-C Induced Cytotoxicity in Four Human Bladder Cancer Cell Lines
- Creators
- Antoine G van der Heijden - Department of Urology, University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The NetherlandsCornelius F.J Jansen - Department of Urology, University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The NetherlandsGerald Verhaegh - Department of Urology, University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The NetherlandsMichael A O’Donnell - Department of Urology, University of Iowa, College of Medicine, Iowa City, IA, USAJack A Schalken - Department of Urology, University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The NetherlandsJ.Alfred Witjes - Department of Urology, University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
- Resource Type
- Journal article
- Publication Details
- European urology, Vol.46(5), pp.670-674
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.eururo.2004.06.009
- PMID
- 15474281
- ISSN
- 0302-2838
- eISSN
- 1873-7560
- Language
- English
- Date published
- 2004
- Academic Unit
- Urology
- Record Identifier
- 9984051778602771
Metrics
17 Record Views