Journal article
The Effect of Peroxynitrite on Sphincter of Oddi Motility
The Journal of surgical research, Vol.81(1), pp.55-58
01/1999
DOI: 10.1006/jsre.1998.5436
PMID: 9889058
Abstract
Background.Nitric oxide (NO•) is an inhibitory neurotransmitter that induces sphincter of Oddi relaxation. Superoxide (O•−2)-scavenging enzymes are present in enteric plexuses of the sphincter of Oddi and O•−2alters sphincter of Oddi motor function. O•−2rapidly oxidizes nitric oxide (NO•) to form peroxynitrite (ONOO−), thus terminating the biological activity of NO•. The aim of our study was to determine the effects of ONOO−on sphincter of Oddi motilityin vitro.
Materials and methods.Adult opossums were sacrificed and the sphincter of Oddi was removed and placed in a tissue bath containing oxygenated Krebs solution at 37°C. In the first series of experiments, force transducers recorded tension in a transverse orientation at two sites along the spontaneously contracting sphincter of Oddi. In a second series of experiments, circular muscle strips were precontracted with carbachol and stimulated by an electrical field.
Results.ONOO−, superoxide dismutase (SOD),Nω-nitro-l-arginine (l-NNA), or oxyhemoglobin were added to the tissue baths. ONOO−decreased the frequency of contractions in the spontaneously contracting sphincter of Oddi. Adding hemoglobin increased the frequency of contractions. ONOO−also increased the stimulation-induced relaxation compared to controls. The increase in relaxation induced by ONOO−was inhibited by oxyhemoglobin andl-NNA but not SOD. Pretreatment with oxyhemoglobin prevented the increase in the stimulation-induced relaxation caused by ONOO−.
Conclusion.These results suggest that hemoglobin binds ONOO−or that ONOO−generates NO.
Details
- Title: Subtitle
- The Effect of Peroxynitrite on Sphincter of Oddi Motility
- Creators
- Brian W HerrmannJoseph J CullenAmber LedlowJoseph A MurrayJeffrey L Conklin
- Resource Type
- Journal article
- Publication Details
- The Journal of surgical research, Vol.81(1), pp.55-58
- Publisher
- Elsevier Inc
- DOI
- 10.1006/jsre.1998.5436
- PMID
- 9889058
- ISSN
- 0022-4804
- eISSN
- 1095-8673
- Language
- English
- Date published
- 01/1999
- Academic Unit
- Surgery; Radiation Oncology
- Record Identifier
- 9984051987702771
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