The Effects of Smoking on Telomere Length, Induction of Oncogenic Stress, and Chronic Inflammatory Responses Leading to Aging

Shreya Deb; Joseph Berei; Edward Miliavski; Muhammad J. Khan; Taylor J. Broder; Thomas A. Akurugo; Cody Lund; Sara E. Fleming; Robert Hillwig; Joseph Ross; Neelu Puri

doi:10.3390/cells13110884

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The Effects of Smoking on Telomere Length, Induction of Oncogenic Stress, and Chronic Inflammatory Responses Leading to Aging

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The Effects of Smoking on Telomere Length, Induction of Oncogenic Stress, and Chronic Inflammatory Responses Leading to Aging

Shreya Deb, Joseph Berei, Edward Miliavski, Muhammad J. Khan, Taylor J. Broder, Thomas A. Akurugo, Cody Lund, Sara E. Fleming, Robert Hillwig, Joseph Ross, …

Cells (Basel, Switzerland), Vol.13(11), p.884

06/01/2024

DOI: 10.3390/cells13110884

PMID: 38891017

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Abstract

Telomeres, potential biomarkers of aging, are known to shorten with continued cigarette smoke exposure. In order to further investigate this process and its impact on cellular stress and inflammation, we used an in vitro model with cigarette smoke extract (CSE) and observed the downregulation of telomere stabilizing TRF2 and POT1 genes after CSE treatment. hTERT is a subunit of telomerase and a well-known oncogenic marker, which is overexpressed in over 85% of cancers and may contribute to lung cancer development in smokers. We also observed an increase in hTERT and ISG15 expression levels after CSE treatment, as well as increased protein levels revealed by immunohistochemical staining in smokers' lung tissue samples compared to non-smokers. The effects of ISG15 overexpression were further studied by quantifying IFN-gamma, an inflammatory protein induced by ISG15, which showed greater upregulation in smokers compared to non-smokers. Similar changes in gene expression patterns for TRF2, POT1, hTERT, and ISG15 were observed in blood and buccal swab samples from smokers compared to non-smokers. The results from this study provide insight into the mechanisms behind smoking causing telomere shortening and how this may contribute to the induction of inflammation and/or tumorigenesis, which may lead to comorbidities in smokers.

Cell Biology

Life Sciences & Biomedicine

Science & Technology

Details

Title: Subtitle: The Effects of Smoking on Telomere Length, Induction of Oncogenic Stress, and Chronic Inflammatory Responses Leading to Aging
Creators: Shreya Deb - University of Illinois Chicago, Rockford campus
Joseph Berei - University of Illinois Chicago, Rockford campus
Edward Miliavski - University of Illinois Chicago, Rockford campus
Muhammad J. Khan - University of Illinois Chicago, Rockford campus
Taylor J. Broder - University of Illinois Chicago, Rockford campus
Thomas A. Akurugo - University of Illinois Chicago, Rockford campus
Cody Lund - University of Illinois Chicago, Rockford campus
Sara E. Fleming - Swedish American Hospital
Robert Hillwig - University of Illinois Chicago, Rockford campus
Joseph Ross - University of Illinois Chicago, Rockford campus
Neelu Puri - University of Illinois Chicago, Rockford campus
Resource Type: Journal article
Publication Details: Cells (Basel, Switzerland), Vol.13(11), p.884
DOI: 10.3390/cells13110884
PMID: 38891017
ISSN: 2073-4409
eISSN: 2073-4409
Publisher: Mdpi
Number of pages: 22
Grant note: University of Illinois College of Medicine at Rockford UICOMR Bridge Grant
Language: English
Date published: 06/01/2024
Academic Unit: Internal Medicine
Record Identifier: 9985218624902771

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