The Epidermolysis Bullosa Acquisita Antigen (Type VII Collagen) is Present in Human Colon and Patients with Crohn's Disease have Autoantibodies to Type VII Collagen

Mei Chen; Edel A O'Toole; Jigisha Sanghavi; David T Woodley; Nasir Mahmud; Dermot Kelleher; Donald Weir; Janet A Fairley

doi:10.1046/j.1523-1747.2002.01772.x

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The Epidermolysis Bullosa Acquisita Antigen (Type VII Collagen) is Present in Human Colon and Patients with Crohn's Disease have Autoantibodies to Type VII Collagen

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The Epidermolysis Bullosa Acquisita Antigen (Type VII Collagen) is Present in Human Colon and Patients with Crohn's Disease have Autoantibodies to Type VII Collagen

Mei Chen, Edel A O'Toole, Jigisha Sanghavi, David T Woodley, Nasir Mahmud, Dermot Kelleher, Donald Weir and Janet A Fairley

Journal of investigative dermatology, Vol.118(6), pp.1059-1064

06/2002

DOI: 10.1046/j.1523-1747.2002.01772.x

PMID: 12060403

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Abstract

Epidermolysis bullosa acquisita is an autoimmune blistering disease of the skin characterized by IgG autoantibodies against type VII collagen. Systemic diseases are often associated with epidermolysis bullosa acquisita, Crohn's disease being the most frequent. This study sought to determine if type VII collagen, the epidermolysis bullosa acquisita autoantigen, was present in normal human colon by western blotting and immunofluorescence. The 290 kDa type VII collagen α chain was demonstrated by western blotting in four normal intraoperative colon specimens. Antibodies to type VII collagen labeled the junction between the intestinal epithelium and the lamina propria. We also used an enzyme-linked immunosorbent assay to test sera from patients with Crohn's disease (n = 19), ulcerative colitis (n = 31), celiac disease (n = 17), rheumatoid arthritis (n = 15), and normal controls (n = 16). It was found that 13 of 19 patients with Crohn's disease and four of 31 patients with ulcerative colitis demonstrated reactivity to type VII collagen. Sera from control subjects, patients with celiac disease or rheumatoid arthritis were negative. The sera from Crohn's disease patients also reacted with type VII collagen by immunoblot analysis. It was concluded that patients with inflammatory bowel disease may have IgG autoantibodies to type VII collagen, which exists in both the skin and the gut.

autoimmunity

skin bullous diseases

inflammatory bowel disease

Details

Title: Subtitle: The Epidermolysis Bullosa Acquisita Antigen (Type VII Collagen) is Present in Human Colon and Patients with Crohn's Disease have Autoantibodies to Type VII Collagen
Creators: Mei Chen
Edel A O'Toole
Jigisha Sanghavi
David T Woodley
Nasir Mahmud - Department of Medicine, St James's Hospital and Trinity College, Dublin, Ireland
Dermot Kelleher - Department of Medicine, St James's Hospital and Trinity College, Dublin, Ireland
Donald Weir - Department of Medicine, St James's Hospital and Trinity College, Dublin, Ireland
Janet A Fairley - Department of Dermatology, Medical College of Wisconsin, Milwaukee, Wisconsin, U.S.A
Resource Type: Journal article
Publication Details: Journal of investigative dermatology, Vol.118(6), pp.1059-1064
Publisher: Elsevier Inc
DOI: 10.1046/j.1523-1747.2002.01772.x
PMID: 12060403
ISSN: 0022-202X
eISSN: 1523-1747
Language: English
Date published: 06/2002
Academic Unit: Dermatology
Record Identifier: 9984025673002771

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