Journal article
The Essential Role of the Choriocapillaris in Vision: Novel Insights from Imaging and Molecular Biology
Annual review of vision science, Vol.8, pp.33-52
09/15/2022
DOI: 10.1146/annurev-vision-100820-085958
PMCID: PMC9668353
PMID: 36108103
Abstract
The choriocapillaris, a dense capillary network located at the posterior pole of the eye, is essential for supporting normal vision, supplying nutrients, and removing waste products from photoreceptor cells and the retinal pigment epithelium. The anatomical location, heterogeneity, and homeostatic interactions with surrounding cell types make the choroid complex to study both in vivo and in vitro. Recent advances in single-cell RNA sequencing, in vivo imaging, and in vitro cell modeling are vastly improving our knowledge of the choroid and its role in normal health and in age-related macular degeneration (AMD). Histologically, loss of endothelial cells (ECs) of the choriocapillaris occurs early in AMD concomitant with elevated formation of the membrane attack complex of complement. Advanced imaging has allowed us to visualize early choroidal blood flow changes in AMD in living patients, supporting histological findings of loss of choroidal ECs. Single-cell RNA sequencing is being used to characterize choroidal cell types transcriptionally and discover their altered patterns of gene expression in aging and disease. Advances in induced pluripotent stem cell protocols and 3D cultures will allow us to closely mimic the in vivo microenvironment of the choroid in vitro to better understand the mechanism leading to choriocapillaris loss in AMD.
Details
- Title: Subtitle
- The Essential Role of the Choriocapillaris in Vision: Novel Insights from Imaging and Molecular Biology
- Creators
- Kelly Mulfaul - University of IowaJonathan F Russell - University of IowaAndrew P Voigt - University of IowaEdwin M Stone - University of IowaBudd A Tucker - University of IowaRobert F Mullins - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Annual review of vision science, Vol.8, pp.33-52
- DOI
- 10.1146/annurev-vision-100820-085958
- PMID
- 36108103
- PMCID
- PMC9668353
- NLM abbreviation
- Annu Rev Vis Sci
- ISSN
- 2374-4642
- eISSN
- 2374-4650
- Publisher
- Annual Reviews
- Language
- English
- Date published
- 09/15/2022
- Academic Unit
- The University of Iowa Institute for Vision Research; Iowa Neuroscience Institute; John and Marcia Carver Nonprofit Genetic Testing Laboratory; Ophthalmology and Visual Sciences
- Record Identifier
- 9984296996702771
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