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The Essential Transcription Factor, Mcm1, Is a Downstream Target of Sln1, a Yeast "Two-component" Regulator
Journal article   Open access   Peer reviewed

The Essential Transcription Factor, Mcm1, Is a Downstream Target of Sln1, a Yeast "Two-component" Regulator

Guoying Yu, Robert J Deschenes and Jan S Fassler
The Journal of biological chemistry, Vol.270(15), pp.8739-8743
04/14/1995
DOI: 10.1074/jbc.270.15.8739
PMID: 7721779
url
https://doi.org/10.1074/jbc.270.15.8739View
Published (Version of record) Open Access

Abstract

In a search for mutants exhibiting altered activity of the yeast transcription factor, Mcm1, we have identified the SLN1 gene, whose product is highly related to bacterial two-component sensor-regulator proteins. sln1 alleles identified in our screen increased Mcm1p-mediated transcriptional activation, while deletion of the SLN1 locus severely reduced Mcm1p activity. Our data establish that Mcm1p is a downstream target of the Sln1 signaling pathway. Yeast Sln1p was recently shown to be involved in osmoregulation and to depend on the Hog1 MAP kinase (Maeda, T., Wurgler-Murphy, S., and Saito, H. (1994) Nature 369, 242-245). We show that SLN1-mediated regulation of Mcm1p activity is independent of the Hog1 MAP kinase, and suggest that the role of SLN1 is not restricted to osmoregulation.

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