Journal article
The Expression of Dystrophin-associated Glycoproteins During Skeletal Muscle Degeneration and Regeneration. An Immunofluorescence Study
Journal of neuropathology and experimental neurology, Vol.54(4), pp.557-569
07/1995
DOI: 10.1097/00005072-199507000-00010
PMID: 7602329
Abstract
The distribution and expression of dystrophin and three of the dystrophin-associated glycoproteins (DAG), α-dystroglycan (156 kDa DAG), β-dystroglycan (43 kDa DAG) and adhalin (50 kDa DAG) in rat skeletal muscle were studied during a controlled cycle of degeneration and regeneration induced by the injection of a snake venom. Cryosections of muscle at various stages of degeneration and regeneration were labeled using monoclonal antibodies to the three glycoproteins and examined at fixed time points after venom injection. Adhalin and α-dystroglycan remained present at the sarcolemma throughout the entire cycle of degeneration and regeneration. β-Dystroglycan, on the other hand, was lost from the sarcolemma by 12 hours and reappeared 2 days after venom injection when new muscle fibers were being formed. Dystrophin was not lost from the sarcolemma until 24 hours after venom injection and did not reappear at the membrane until 4 days. It is suggested that dystrophin and the glycoprotein complex are synthesized separately, both temporally and spatially, and only become associated at the plasma membrane during the later stages of regeneration. The expression of β-dystroglycan in the regenerating muscle fibers was first seen at sites of newly forming plasma membrane that were closely associated with the old basal lamina tube. The basal lamina may therefore have a regulatory or modulatory role in the expression of the DAG.
Details
- Title: Subtitle
- The Expression of Dystrophin-associated Glycoproteins During Skeletal Muscle Degeneration and Regeneration. An Immunofluorescence Study
- Creators
- RUTH VATER - From the Muscular Dystrophy Group Research Laboratories, Regional Neurosciences Centre, Newcastle General Hospital, Newcastle upon Tyne, United Kingdom (RV, JBH, LVBA, MJC), and the Howard Hughes Medical Institute and Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa (SLR, KPC)JOHN HARRISLOUISE ANDERSONSTEVEN ROBERDSKEVIN CAMPBELLMICHAEL CULLEN
- Resource Type
- Journal article
- Publication Details
- Journal of neuropathology and experimental neurology, Vol.54(4), pp.557-569
- DOI
- 10.1097/00005072-199507000-00010
- PMID
- 7602329
- NLM abbreviation
- J Neuropathol Exp Neurol
- ISSN
- 0022-3069
- eISSN
- 1554-6578
- Publisher
- American Association of Neuropathologists, Inc
- Language
- English
- Date published
- 07/1995
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068379502771
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