Journal article
The Host Antimicrobial Protein Calgranulin C Participates in the Control of Campylobacter jejuni Growth via Zinc Sequestration
Infection and immunity, Vol.86(6), 00234
06/01/2018
DOI: 10.1128/IAI.00234-18
PMCID: PMC5964530
PMID: 29610259
Abstract
Campylobacter jejuni is a leading cause of bacterially derived gastroenteritis worldwide. Campylobacter is most commonly acquired through the consumption of undercooked poultry meat or through drinking contaminated water. Following ingestion, Campylobacter adheres to the intestinal epithelium and mucus layer, causing toxin-mediated inflammation and inhibition of fluid reabsorption. Currently, the human response to infection is relatively unknown, and animal hosts that model these responses are rare. As such, we examined patient fecal samples for the accumulation of the neutrophil protein calgranulin C during infection with Campylobacter jejuni. In response to infection, calgranulin C was significantly increased in the feces of humans. To determine whether calgranulin C accumulation occurs in an animal model, we examined disease in ferrets. Ferrets were effectively infected by C. jejuni, with peak fecal loads observed at day 3 postinfection and full resolution by day 12. Serum levels of interleukm-10 (IL-10) and tumor necrosis factor alpha (TNF-alpha) significantly increased in response to infection, which resulted in leukocyte trafficking to the colon. As a result, calgranulin C increased in the feces of ferrets at the time when C. jejuni loads decreased. Further, the addition of purified calgranulin C to C. jejuni cultures was found to inhibit growth in a zinc-dependent manner. These results suggest that upon infection with C. jejuni, leukocytes trafficked to the intestine release calgranulin C as a mechanism for inhibiting C. jejuni growth.
Details
- Title: Subtitle
- The Host Antimicrobial Protein Calgranulin C Participates in the Control of Campylobacter jejuni Growth via Zinc Sequestration
- Creators
- Janette M. Shank - University of Tennessee at KnoxvilleBrittni R. Kelley - University of Tennessee at KnoxvilleJoseph W. Jackson - University of Tennessee at KnoxvilleJessica L. Tweedie - University of Tennessee at KnoxvilleDana Franklin - Fisk UniversitySteven M. Damo - Fisk UniversityJennifer A. Gaddy - Vanderbilt University Medical CenterCaitlin N. Murphy - University of Nebraska Medical CenterJeremiah G. Johnson - University of Tennessee at Knoxville
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.86(6), 00234
- Publisher
- Amer Soc Microbiology
- DOI
- 10.1128/IAI.00234-18
- PMID
- 29610259
- PMCID
- PMC5964530
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Number of pages
- 14
- Grant note
- R01 HD090061 / National Institutes of Health (NICHD); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) UL1RR024975 / NATIONAL CENTER FOR RESEARCH RESOURCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) 2 UL1 TR000445-06 / National Center for Advancing Translational Sciences; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS) R01HD090061 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) UL1TR000445 / NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS) P30DK058404 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA IK2BX001701 / Veterans Affairs; US Department of Veterans Affairs P30DK058404 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) University of Tennessee IK2BX001701 / Office of Medical Research, Department of Veterans Affairs; US Department of Veterans Affairs Vanderbilt University Medical Center's Digestive Disease Research Center UL1 RR024975-01 / National Center for Research Resources; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) HRD1547757 / National Science Foundation; National Science Foundation (NSF) Digestive Disease Research Center Vanderbilt Institute for Clinical and Translational Research Program
- Language
- English
- Date published
- 06/01/2018
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696720802771
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