Journal article
The Hsp72 and Hsp90 alpha mRNA Responses to Hot Downhill Running Are Reduced Following a Prior Bout of Hot Downhill Running, and Occur Concurrently within Leukocytes and the Vastus Lateralis
Frontiers in physiology, Vol.8, pp.473-473
07/12/2017
DOI: 10.3389/fphys.2017.00473
PMCID: PMC5506191
PMID: 28747888
Abstract
The leukocyte heat shock response (HSR) is used to determine individual's thermotolerance. The HSR and thermotolerance are enhanced following interventions such as preconditioning and/or acclimation/acclimatization. However, it is unclear whether the leukocyte HSR is an appropriate surrogate for the HSR in other tissues implicated within the pathophysiology of exertional heat illnesses (e.g., skeletal muscle), and whether an acute preconditioning strategy (e.g., downhill running) can improve subsequent thermotolerance. Physically active, non-heat acclimated participants were split into two groups to investigate the benefits of hot downhill running as preconditioning strategy. A hot preconditioning group (HPC; n = 6) completed two trials (HpPC1(HOTDOWN) and HPC2(HOTDOWN)) of 30 min running at lactate threshold (LT) on -10% gradient in 30 degrees C and 50% relative humidity (RH) separated by 7 d. A temperate preconditioning group (TPC; n = 5) completed 30 min running at LT on a - 1% gradient in 20 degrees C and 50% (TPC1(TEMPFLAT)) and 7 d later completed 30 min running at LT on -10% gradient in 30. C and 50% RH (TPC2(HOTDOWN)). Venous blood samples and muscle biopsies (vastus lateralis; VL) were obtained before, immediately after, 3, 24, and 48 h after each trial. Leukocyte and VL Hsp72, Hsp90 a, and Grp78 mRNA relative expression was determined via RT-QPCR. Attenuated leukocyte and VL Hsp72 (2.8 to 1.8 fold and 5.9 to 2.4 fold; p < 0.05) and Hsp90 a mRNA (2.9 to 2.4 fold and 5.2 to 2.4 fold; p < 0.05) responses accompanied reductions (p < 0.05) in physiological strain [ exercising rectal temperature (-0.3 degrees C) and perceived muscle soreness (similar to - 14%)] during HPC2(HOTDOWN) compared to HPC1(HOTDOWN) (i.e., a preconditioning effect). Both VL and leukocyte Hsp72 and Hsp90 a mRNA increased (p < 0.05) simultaneously following downhill runs and demonstrated a strong relationship (p < 0.01) of similar magnitudes with one another. Hot downhill running is an effective preconditioning strategy which ameliorates physiological strain, soreness and Hsp72 and Hsp90 a mRNA responses to a subsequent bout. Leukocyte and VL analyses are appropriate tissues to infer the extent to which the HSR has been augmented.
Details
- Title: Subtitle
- The Hsp72 and Hsp90 alpha mRNA Responses to Hot Downhill Running Are Reduced Following a Prior Bout of Hot Downhill Running, and Occur Concurrently within Leukocytes and the Vastus Lateralis
- Creators
- James A. Tuttle - University of BedfordshireBryna C. R. Chrismas - Florida State UniversityOliver R. Gibson - Brunel University of LondonJames H. Barrington - University of BedfordshireDavid C. Hughes - University of California, DavisPaul C. Castle - University of BedfordshireAlan J. Metcalfe - University of BedfordshireAdrian W. Midgley - Edge Hill UniversityOliver Pearce - Milton Keynes HospitalChindu Kabir - Milton Keynes HospitalFaizal Rayanmarakar - Milton Keynes HospitalSami Al-Ali - Milton Keynes HospitalMark P. Lewis - Loughborough UniversityLee Taylor - Qatar Orthopaedic and Sports Medicine Hospital
- Resource Type
- Journal article
- Publication Details
- Frontiers in physiology, Vol.8, pp.473-473
- DOI
- 10.3389/fphys.2017.00473
- PMID
- 28747888
- PMCID
- PMC5506191
- NLM abbreviation
- Front Physiol
- ISSN
- 1664-042X
- eISSN
- 1664-042X
- Publisher
- Frontiers Media Sa
- Number of pages
- 15
- Language
- English
- Date published
- 07/12/2017
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984359680302771
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