Journal article
The Ih Channel Gene Promotes Synaptic Transmission and Coordinated Movement in Drosophila melanogaster
Frontiers in molecular neuroscience, Vol.10, pp.41-41
02/24/2017
DOI: 10.3389/fnmol.2017.00041
PMCID: PMC5323408
PMID: 28286469
Abstract
Hyperpolarization-activated cyclic nucleotide-gated “HCN” channels, which underlie the hyperpolarization-activated current (I
h
), have been proposed to play diverse roles in neurons. The presynaptic HCN channel is thought to both promote and inhibit neurotransmitter release from synapses, depending upon its interactions with other presynaptic ion channels. In larvae of
Drosophila melanogaster
, inhibition of the presynaptic HCN channel by the drug ZD7288 reduces the enhancement of neurotransmitter release at motor terminals by serotonin but this drug has no effect on basal neurotransmitter release, implying that the channel does not contribute to firing under basal conditions. Here, we show that genetic disruption of the sole HCN gene
(Ih)
reduces the amplitude of the evoked response at the neuromuscular junction (NMJ) of third instar larvae by decreasing the number of released vesicles. The anatomy of the (NMJ) is not notably affected by disruption of the
Ih
gene. We propose that the presynaptic HCN channel is active under basal conditions and promotes neurotransmission at larval motor terminals. Finally, we demonstrate that
Ih
partial loss-of-function mutant adult flies have impaired locomotion, and, thus, we hypothesize that the presynaptic HCN channel at the (NMJ) may contribute to coordinated movement.
Details
- Title: Subtitle
- The Ih Channel Gene Promotes Synaptic Transmission and Coordinated Movement in Drosophila melanogaster
- Creators
- Andrew P Hegle - Department of Cellular and Physiological Sciences, The University of British ColumbiaC. Andrew Frank - Department of Anatomy and Cell Biology, The University of IowaAnthony Berndt - Department of Cellular and Physiological Sciences, The University of British ColumbiaMarkus Klose - Department of Cellular and Physiological Sciences, The University of British ColumbiaDouglas W Allan - Department of Cellular and Physiological Sciences, The University of British ColumbiaEric A Accili - Department of Cellular and Physiological Sciences, The University of British Columbia
- Resource Type
- Journal article
- Publication Details
- Frontiers in molecular neuroscience, Vol.10, pp.41-41
- DOI
- 10.3389/fnmol.2017.00041
- PMID
- 28286469
- PMCID
- PMC5323408
- NLM abbreviation
- Front Mol Neurosci
- ISSN
- 1662-5099
- eISSN
- 1662-5099
- Publisher
- Frontiers Media S.A
- Grant note
- Canadian Institutes of Health Research University of Iowa National Institutes of Health Natural Sciences and Engineering Research Council of Canada
- Language
- English
- Date published
- 02/24/2017
- Academic Unit
- Anatomy and Cell Biology; Iowa Neuroscience Institute
- Record Identifier
- 9984025303402771
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