Journal article
The Immune Response Modifiers Imiquimod and R-848 Are Potent Activators of B Lymphocytes
Cellular immunology, Vol.203(1), pp.55-65
07/10/2000
DOI: 10.1006/cimm.2000.1673
PMID: 10915562
Abstract
Imiquimod and R-848 are members of a family of immune response modifiers that stimulate cytokine production in monocyte/macrophages and dendritic cell cultures. This study evaluated the effects of the imidazoquinolines, imiquimod and R-848, on B lymphocyte activation. Both agents induced proliferation of murine T-cell-depleted and highly purified splenic B cell preparations as well as purified human B cells. Resting and activated B cells responded to these agents, with activated cells responding more efficiently. B cells from the LPS-hyporesponsive C3H/HeJ mice and guanosine-hyporesponsive SJL mice proliferated in response to imiquimod and R-848, indicating a different mechanism of action than lipopolysaccharide and guanine nucleosides. B cells were also stimulated by imiquimod and R-848 to produce increased immunoglobulin levels. Increased expression of a number of B cell activation markers were seen following imiquimod or R-848 stimulation. Finally, R-848 was shown to act as a vaccine adjuvant enhancing OVA-specific IgG2a levels while suppressing total IgE. These results indicate that R-848 and imiquimod are potent activators of B lymphocytes and are capable of augmenting antigen-specific immunoglobulin production.
Details
- Title: Subtitle
- The Immune Response Modifiers Imiquimod and R-848 Are Potent Activators of B Lymphocytes
- Creators
- Mark A Tomai - Department of Pharmacology, 3M Pharmaceuticals, 3M Center, St. Paul, Minnesota, 55144Linda M Imbertson - Department of Pharmacology, 3M Pharmaceuticals, 3M Center, St. Paul, Minnesota, 55144Tamara L Stanczak - Department of Pharmacology, 3M Pharmaceuticals, 3M Center, St. Paul, Minnesota, 55144Lorraine T Tygrett - Department of Pathology, University of Iowa, Iowa City, Iowa, 52242Thomas J Waldschmidt - Department of Pathology, University of Iowa, Iowa City, Iowa, 52242
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.203(1), pp.55-65
- Publisher
- Elsevier Inc
- DOI
- 10.1006/cimm.2000.1673
- PMID
- 10915562
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 07/10/2000
- Academic Unit
- Pathology
- Record Identifier
- 9984047705402771
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