Journal article
The MicroRNA-Processing Enzyme Dicer Maintains Juxtaglomerular Cells
Journal of the American Society of Nephrology, Vol.21(3), pp.460-467
03/01/2010
DOI: 10.1681/ASN.2009090964
PMCID: PMC2831866
PMID: 20056748
Abstract
Juxtaglomerular cells are highly specialized myoepithelioid granulated cells located in the glomerular afferent arterioles. These cells synthesize and release renin, which distinguishes them from other cells. How these cells maintain their identity, restricted localization, and fate is unknown and is fundamental to the control of BP and homeostasis of fluid and electrolytes. Because microRNAs may control cell fate
via
temporal and spatial gene regulation, we generated mice with a conditional deletion of
Dicer
, the RNase III endonuclease that produces mature microRNAs in cells of the renin lineage. Deletion of
Dicer
severely reduced the number of juxtaglomerular cells, decreased expression of the renin genes (
Ren1
and
Ren2
), lowered plasma renin concentration, and decreased BP. As a consequence of the disappearance of renin-producing cells, the kidneys developed striking vascular abnormalities and prominent striped fibrosis. We conclude that microRNAs maintain the renin-producing juxtaglomerular cells and the morphologic integrity and function of the kidney.
Details
- Title: Subtitle
- The MicroRNA-Processing Enzyme Dicer Maintains Juxtaglomerular Cells
- Creators
- Maria Luisa S. Sequeira-Lopez - University of VirginiaEric T. Weatherford - University of IowaGiulianna R. Borges - University of IowaMaria C. Monteagudo - University of VirginiaEllen S. Pentz - University of VirginiaBrian D. Harfe - University of Florida HealthOscar Carretero - Hypertension InstituteCurt D. Sigmund - University of IowaR. Ariel Gomez - University of Virginia
- Resource Type
- Journal article
- Publication Details
- Journal of the American Society of Nephrology, Vol.21(3), pp.460-467
- Publisher
- American Society of Nephrology
- DOI
- 10.1681/ASN.2009090964
- PMID
- 20056748
- PMCID
- PMC2831866
- ISSN
- 1046-6673
- eISSN
- 1533-3450
- Language
- English
- Date published
- 03/01/2010
- Academic Unit
- Molecular Physiology and Biophysics; Neuroscience and Pharmacology; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984359777502771
Metrics
10 Record Views