Journal article
The NMR Structure of Human β-Defensin-2 Reveals a Novel α-Helical Segment
Biochemistry (Easton), Vol.40(13), pp.3810-3816
04/03/2001
DOI: 10.1021/bi002519d
PMID: 11300761
Abstract
Human beta-defensin-2 (HBD-2) is a member of the defensin family of antimicrobial peptides. HBD-2 was first isolated from inflamed skin where it is posited to participate in the killing of invasive bacteria and in the recruitment of cells of the adaptive immune response. Static light scattering and two-dimensional proton nuclear magnetic resonance spectroscopy have been used to assess the physical state and structure of HBD-2 in solution. At concentrations of < or = 2.4 mM, HBD-2 is monomeric. The structure is amphiphilic with a nonuniform surface distribution of positive charge and contains several key structural elements, including a triple-stranded, antiparallel beta-sheet with strands 2 and 3 in a beta-hairpin conformation. A beta-bulge in the second strand occurs at Gly28, a position conserved in the entire defensin family. In solution, HBD-2 exhibits an alpha-helical segment near the N-terminus that has not been previously ascribed to solution structures of alpha-defensins or to the beta-defensin BNBD-12. This novel structural element may be a factor contributing to the specific microbicidal or chemokine-like properties of HBD-2.
Details
- Title: Subtitle
- The NMR Structure of Human β-Defensin-2 Reveals a Novel α-Helical Segment
- Creators
- Monali V SAWAI - Departments of Microbiology and Pediatrics and NMR Facility, The University of Iowa College of Medicine,Iowa City, Iowa 52242, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242,and Department of Medicine, University of California School of Medicine, Los Angeles, California 90095Hong Peng JIA - Departments of Microbiology and Pediatrics and NMR Facility, The University of Iowa College of Medicine,Iowa City, Iowa 52242, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242,and Department of Medicine, University of California School of Medicine, Los Angeles, California 90095Lide LIU - Departments of Microbiology and Pediatrics and NMR Facility, The University of Iowa College of Medicine,Iowa City, Iowa 52242, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242,and Department of Medicine, University of California School of Medicine, Los Angeles, California 90095Vladimir ASEYEV - Departments of Microbiology and Pediatrics and NMR Facility, The University of Iowa College of Medicine,Iowa City, Iowa 52242, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242,and Department of Medicine, University of California School of Medicine, Los Angeles, California 90095John M WIENCEK - Departments of Microbiology and Pediatrics and NMR Facility, The University of Iowa College of Medicine,Iowa City, Iowa 52242, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242,and Department of Medicine, University of California School of Medicine, Los Angeles, California 90095Paul B MCCRAY - Departments of Microbiology and Pediatrics and NMR Facility, The University of Iowa College of Medicine,Iowa City, Iowa 52242, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242,and Department of Medicine, University of California School of Medicine, Los Angeles, California 90095Tomas GANZ - Departments of Microbiology and Pediatrics and NMR Facility, The University of Iowa College of Medicine,Iowa City, Iowa 52242, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242,and Department of Medicine, University of California School of Medicine, Los Angeles, California 90095William R KEARNEY - Departments of Microbiology and Pediatrics and NMR Facility, The University of Iowa College of Medicine,Iowa City, Iowa 52242, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242,and Department of Medicine, University of California School of Medicine, Los Angeles, California 90095Brian F TACK - Departments of Microbiology and Pediatrics and NMR Facility, The University of Iowa College of Medicine,Iowa City, Iowa 52242, Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242,and Department of Medicine, University of California School of Medicine, Los Angeles, California 90095
- Resource Type
- Journal article
- Publication Details
- Biochemistry (Easton), Vol.40(13), pp.3810-3816
- Publisher
- American Chemical Society
- DOI
- 10.1021/bi002519d
- PMID
- 11300761
- ISSN
- 0006-2960
- eISSN
- 1520-4995
- Language
- English
- Date published
- 04/03/2001
- Academic Unit
- Radiology; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984093597802771
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