Journal article
The Neuropathic Diabetic Foot Ulcer Microbiome is Associated with Clinical Factors.
Diabetes, Vol.62(3), pp.923-930
03/01/2013
DOI: 10.2337/db12-0771
PMID: 23139351
Abstract
Nonhealing diabetic foot ulcers (DFUs) are a common and costly complication of diabetes. Microbial burden, or "bioburden," is believed to underlie delayed healing, although little is known of those clinical factors that may influence microbial load, diversity, and/or pathogenicity. We profiled the microbiomes of neuropathic nonischemic DFUs without clinical evidence of infection in 52 individuals using high-throughput sequencing of the bacterial 16S ribosomal RNA gene. Comparatively, wound cultures, the standard diagnostic in the clinic, vastly underrepresent microbial load, microbial diversity, and the presence of potential pathogens. DFU microbiomes were heterogeneous, even in our tightly restricted study population, but partitioned into three clusters distinguished primarily by dominant bacteria and diversity. Ulcer depth was associated with ulcer cluster, positively correlated with abundance of anaerobic bacteria, and negatively correlated with abundance of Staphylococcus. Ulcer duration was positively correlated with bacterial diversity, species richness, and relative abundance of Proteobacteria, but was negatively correlated with relative abundance of Staphylococcus. Finally, poor glycemic control was associated with ulcer cluster, with poorest median glycemic control concentrating to Staphylococcus-rich and Streptococcus-rich ulcer clusters. Analyses of microbial community membership and structure may provide the most useful metrics in prospective studies to delineate problematic bioburden from benign colonization that can then be used to drive clinical treatment.
Details
- Title: Subtitle
- The Neuropathic Diabetic Foot Ulcer Microbiome is Associated with Clinical Factors.
- Creators
- Sue E. Gardner - University of IowaStephen L. HillisKris HeilmannJulia A. SegreElizabeth A. Grice
- Resource Type
- Journal article
- Publication Details
- Diabetes, Vol.62(3), pp.923-930
- Publisher
- American Diabetes Association
- DOI
- 10.2337/db12-0771
- PMID
- 23139351
- ISSN
- 1939-327X
- Copyright
- © 2013 by the American Diabetes Association
- Language
- English
- Date published
- 03/01/2013
- Academic Unit
- Nursing
- Record Identifier
- 9983557665602771
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