Journal article
The O-glycosylated Stalk Domain Is Required for Apical Sorting of Neurotrophin Receptors in Polarized MDCK Cells
The Journal of cell biology, Vol.139(4), pp.929-940
11/17/1997
DOI: 10.1083/jcb.139.4.929
PMCID: PMC2139957
PMID: 9362511
Abstract
Delivery of newly synthesized membrane-spanning proteins to the apical plasma membrane domain of polarized MDCK epithelial cells is dependent on yet unidentified sorting signals present in the luminal domains of these proteins. In this report we show that structural information for apical sorting of transmembrane neurotrophin receptors (p75NTR) is localized to a juxtamembrane region of the extracellular domain that is rich in O-glycosylated serine/threonine residues. An internal deletion of 50 amino acids that removes this stalk domain from p75NTR causes the protein to be sorted exclusively of the basolateral plasma membrane. Basolateral sorting stalk-minus p75NTR does not occur by default, but requires sequences present in the cytoplasmic domain. The stalk domain is also required for apical secretion of a soluble form of p75NTR, providing the first demonstration that the same domain can mediate apical sorting of both a membrane-anchored as well as secreted protein. However, the single N-glycan present on p75NTR is not required for apical sorting of either transmembrane or secreted forms.
Details
- Title: Subtitle
- The O-glycosylated Stalk Domain Is Required for Apical Sorting of Neurotrophin Receptors in Polarized MDCK Cells
- Creators
- Charles Yeaman - Dyson Vision Research Institute, Department of Ophthalmology, and Department of Cell Biology, Cornell University Medical College, New York 10021; Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305; and Laboratoire de Genetique et Physiologie du Developpement, Unite Mixte de Recherche 9943 Faculte des Sciences de Luminy, 13288 Marseille, Cedex 09 FranceAnnick H. Le Gall - Dyson Vision Research Institute, Department of Ophthalmology, and Department of Cell Biology, Cornell University Medical College, New York 10021; Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305; and Laboratoire de Genetique et Physiologie du Developpement, Unite Mixte de Recherche 9943 Faculte des Sciences de Luminy, 13288 Marseille, Cedex 09 FranceAnne N Baldwin - Dyson Vision Research Institute, Department of Ophthalmology, and Department of Cell Biology, Cornell University Medical College, New York 10021; Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305; and Laboratoire de Genetique et Physiologie du Developpement, Unite Mixte de Recherche 9943 Faculte des Sciences de Luminy, 13288 Marseille, Cedex 09 FranceLaure Monlauzeur - Dyson Vision Research Institute, Department of Ophthalmology, and Department of Cell Biology, Cornell University Medical College, New York 10021; Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305; and Laboratoire de Genetique et Physiologie du Developpement, Unite Mixte de Recherche 9943 Faculte des Sciences de Luminy, 13288 Marseille, Cedex 09 FranceAndre Le Bivic - Dyson Vision Research Institute, Department of Ophthalmology, and Department of Cell Biology, Cornell University Medical College, New York 10021; Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305; and Laboratoire de Genetique et Physiologie du Developpement, Unite Mixte de Recherche 9943 Faculte des Sciences de Luminy, 13288 Marseille, Cedex 09 FranceEnrique Rodriguez-Boulan - Dyson Vision Research Institute, Department of Ophthalmology, and Department of Cell Biology, Cornell University Medical College, New York 10021; Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305; and Laboratoire de Genetique et Physiologie du Developpement, Unite Mixte de Recherche 9943 Faculte des Sciences de Luminy, 13288 Marseille, Cedex 09 France
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.139(4), pp.929-940
- DOI
- 10.1083/jcb.139.4.929
- PMID
- 9362511
- PMCID
- PMC2139957
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 11/17/1997
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025324602771
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