Journal article
The Onset of CD8+-T-Cell Contraction Is Influenced by the Peak of Listeria monocytogenes Infection and Antigen Display
Infection and immunity, Vol.74(3), pp.1528-1536
03/2006
DOI: 10.1128/IAI.74.3.1528-1536.2006
PMCID: PMC1418632
PMID: 16495523
Abstract
The CD8
+
-T-cell response to infection with
Listeria monocytogenes
consists of expansion, contraction, and memory phases. The transition between expansion and contraction is reported to occur on different days postinfection with virulent (8 to 9 days) and attenuated (Δ
actA
) (7 days)
L. monocytogenes
strains. We hypothesized that differences in the infectious courses, and therefore antigen (Ag) display, determine the precise time of the expansion/contraction transition in response to these infections. To test this, we infected BALB/c mice with 0.1 50% lethal dose of Δ
actA
or virulent
L. monocytogenes
and measured bacterial numbers, Ag display, and Ag-specific CD8
+
-T-cell responses on various days after infection. We found that bacterial numbers and Ag display peaked between 12 and 36 h and between 36 and 60 h after infection with Δ
actA
and virulent
L. monocytogenes
strains, respectively. Infection with Δ
actA L. monocytogenes
resulted in a sharp peak in the Ag-specific CD8
+
-T-cell response on day 7, while infection with virulent
L. monocytogenes
yielded a prolonged peak with equivalent numbers of Ag-specific CD8
+
T cells on days 6, 7, and 8 after infection. Truncating virulent infection with antibiotics on day 1 or 2 after infection resulted in a shift in the expansion/contraction transition from day 8 to day 7 after infection. However, antibiotic treatment beginning on day 3, after the peak of virulent
L. monocytogenes
infection and Ag display, had no effect upon the magnitude or timing of the CD8
+
-T-cell response. These results demonstrate a direct relationship between the course of infection and Ag display and that the timing of these events is important in shaping the T-cell response to infection.
Details
- Title: Subtitle
- The Onset of CD8+-T-Cell Contraction Is Influenced by the Peak of Listeria monocytogenes Infection and Antigen Display
- Creators
- Brandon B Porter - Interdisciplinary Program in ImmunologyJohn T Harty - Interdisciplinary Program in Immunology
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.74(3), pp.1528-1536
- DOI
- 10.1128/IAI.74.3.1528-1536.2006
- PMID
- 16495523
- PMCID
- PMC1418632
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 03/2006
- Academic Unit
- Pathology
- Record Identifier
- 9984047612902771
Metrics
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