Journal article
The Oxygen Radical Scavenger Pyrrolidine Dithiocarbamate Enhances Interleukin-1β-Induced Cyclooxygenase-2 Expression in Cerebral Microvascular Smooth Muscle Cells
Microvascular research, Vol.64(3), pp.405-413
2002
DOI: 10.1006/mvre.2002.2431
PMID: 12453435
Abstract
Oxidative stress and inducible cyclooxygenase-2 (COX-2)-mediated prostaglandin (PG) formation have been proposed to play an important role in cytokine-induced vascular pathology. To explore the relationship between oxidative stress and COX-2 induction, cultured murine cerebral microvascular smooth muscle cells (SMCs) were stimulated with interleukin-1β (IL-1β) in the presence or absence of an oxygen radical scavenger, pyrrolidine dithiocarbamate (PDTC). IL-1β increased COX-2 protein expression in a dose- and time-dependent manner, an increase that was further enhanced by PDTC in a dose-dependent manner. PDTC did not, however, affect the expression of COX-1 protein. In the presence of 100 μM PDTC, PGE
2 production induced by IL-1β (5 ng/ml) was increased by threefold as compared with IL-1β alone. Although PDTC enhanced COX-2 protein expression, it did not increase IL-1β-induced expression of COX-2 mRNA, indicating that the regulatory effect occurred at the posttranscriptional level. The time course of COX-2 protein degradation indicated that PDTC also did not alter the stability of the COX-2 protein induced by IL-1β. These results suggest that endogenous oxygen radicals may blunt COX-2 induced by IL-1β through an effect on translation.
Details
- Title: Subtitle
- The Oxygen Radical Scavenger Pyrrolidine Dithiocarbamate Enhances Interleukin-1β-Induced Cyclooxygenase-2 Expression in Cerebral Microvascular Smooth Muscle Cells
- Creators
- Xiang Fang - Department of Biochemistry, University of Iowa College of Medicine, Iowa City, Iowa, 52242Ping Chen - Department of Biochemistry, University of Iowa College of Medicine, Iowa City, Iowa, 52242Steven A Moore - Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa, 52242
- Resource Type
- Journal article
- Publication Details
- Microvascular research, Vol.64(3), pp.405-413
- Publisher
- Elsevier Inc
- DOI
- 10.1006/mvre.2002.2431
- PMID
- 12453435
- ISSN
- 0026-2862
- eISSN
- 1095-9319
- Language
- English
- Date published
- 2002
- Academic Unit
- Pathology
- Record Identifier
- 9984047731302771
Metrics
22 Record Views