Journal article
The Pitx2 protein in mouse development
Developmental dynamics, Vol.218(1), pp.195-200
05/2000
DOI: 10.1002/(SICI)1097-0177(200005)218:1<195::AID-DVDY17>3.0.CO;2-C
PMID: 10822271
Abstract
The Rieger syndrome, an autosomal dominant disorder involving ocular, dental, and umbilical defects is caused by mutations in PITX2, a Bicoid‐type homeobox protein. Mouse Pitx2 mRNA is expressed in eye, tooth and umbilicus consistent with the human Riegers phenotype. Moreover, Pitx2 is involved in the Nodal/Sonic hedgehog pathway that determines left/right polarity. In this report we demonstrate a 32‐kDa polypeptide on Western blots of nuclear extracts from a rat pituitary cell line, using a Pitx2 specific antibody (designated P2R10). We describe also for the first time expression of the Pitx2 protein in mouse. Pitx2 protein immunostaining was detectable during the development of the eye, tooth, umbilicus, and also in the pituitary, heart, gut, and limb. We demonstrate for the first time directly that Pitx2 is asymmetrically expressed in early heart, gut, and lung development. Dev Dyn;218:195–200. © 2000 Wiley‐Liss, Inc.
Details
- Title: Subtitle
- The Pitx2 protein in mouse development
- Creators
- Tord A HjaltElena V SeminaBrad A AmendtJeffrey C Murray
- Resource Type
- Journal article
- Publication Details
- Developmental dynamics, Vol.218(1), pp.195-200
- DOI
- 10.1002/(SICI)1097-0177(200005)218:1<195::AID-DVDY17>3.0.CO;2-C
- PMID
- 10822271
- NLM abbreviation
- Dev Dyn
- ISSN
- 1058-8388
- eISSN
- 1097-0177
- Publisher
- John Wiley & Sons, Inc; New York
- Number of pages
- 6
- Grant note
- National Institutes of Health (DE‐09170; EY‐12384) Fight for Sight, Prevent Blindness America (PD99018)
- Language
- English
- Date published
- 05/2000
- Academic Unit
- Orthodontics; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Epidemiology; Pediatric Dentistry; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984025357202771
Metrics
22 Record Views