Journal article
The Pitx2c N-Terminal Domain Is a Critical Interaction Domain Required for Asymmetric Morphogenesis
Developmental dynamics, Vol.238(10), pp.2459-2470
10/01/2009
DOI: 10.1002/dvdy.22062
PMCID: PMC3014603
PMID: 19681163
Abstract
The paired-like homeodomain transcription factor Pitx2c has an essential role in patterning the left-right axis. However, neither its transcriptional targets nor the molecular mechanisms through which it exerts its patterning function are known. Here we provide evidence that the N-terminal domain of Pitx2c is important for this activity. Overexpression of the Pitx2c N-terminus in ovo randomizes the direction of heart looping, the first morphological asymmetry conserved in vertebrate embryos. In addition, the Pitx2c N-terminal domain blocks the ability of Pitx2c to synergize with Nkx2.5 to transactivate the procollagen lysyl hydroxylase (Plod-1) promoter in transient transfection assays. A five amino acid region containing leucine-41 is required for both of these effects. Our data suggest that the Pitx2c N-terminal domain competes with endogenous Pitx2c for binding to a protein interaction partner that is required for the activation of genes that direct asymmetric morphogenesis along the left-right axis. Developmental Dynamics 238.2459-2470, 2009. (c) 2009 Wiley-Liss, Inc.
Details
- Title: Subtitle
- The Pitx2c N-Terminal Domain Is a Critical Interaction Domain Required for Asymmetric Morphogenesis
- Creators
- Annie Simard - McGill UniversityLuciano Di Giorgio - McGill UniversityMelanie Amen - Texas A&M Health Science CenterAshley Westwood - Texas A&M Health Science CenterBrad A. Amendt - Texas A&M Health Science CenterAimee K. Ryan - McGill University
- Resource Type
- Journal article
- Publication Details
- Developmental dynamics, Vol.238(10), pp.2459-2470
- DOI
- 10.1002/dvdy.22062
- PMID
- 19681163
- PMCID
- PMC3014603
- NLM abbreviation
- Dev Dyn
- ISSN
- 1058-8388
- eISSN
- 1097-0177
- Publisher
- Wiley
- Number of pages
- 12
- Grant note
- DE 13941 / National Institutes of Health-National Institute of Dental and Craniofacial Research; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) R01DE013941 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) MOP-84583 / Canadian Institutes of Health Research; Canadian Institutes of Health Research (CIHR)
- Language
- English
- Date published
- 10/01/2009
- Academic Unit
- Orthodontics; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Dental Research
- Record Identifier
- 9984284330302771
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