Journal article
The RecA Binding Locus of RecBCD Is a General Domain for Recruitment of DNA Strand Exchange Proteins
Molecular cell, Vol.21(4), pp.573-580
2006
DOI: 10.1016/j.molcel.2006.01.007
PMID: 16483938
Abstract
RecBCD enzyme facilitates loading of RecA protein onto ssDNA produced by its helicase/nuclease activity. This process is essential for RecBCD-mediated homologous recombination. Here, we establish that the C-terminal nuclease domain of the RecB subunit (RecB
nuc) forms stable complexes with RecA. Interestingly, RecB
nuc also interacts with and loads noncognate DNA strand exchange proteins. Interaction is with a conserved element of the RecA-fold, but because the binding to noncognate proteins decreases in a phylogenetically consistent way, species-specific interactions are also present. RecB
nuc does not impede activities of RecA that are important to DNA strand exchange, consistent with its role in targeting of RecA. Modeling predicts the interaction interface for the RecA-RecBCD complex. Because a similar interface is involved in the binding of human Rad51 to the conserved BRC repeat of BRCA2 protein, the RecB-domain may be one of several structural domains that interact with and recruit DNA strand exchange proteins to DNA.
Details
- Title: Subtitle
- The RecA Binding Locus of RecBCD Is a General Domain for Recruitment of DNA Strand Exchange Proteins
- Creators
- Maria Spies - Sections of Microbiology and of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, Davis, California 95616Stephen C Kowalczykowski - Sections of Microbiology and of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, Davis, California 95616
- Resource Type
- Journal article
- Publication Details
- Molecular cell, Vol.21(4), pp.573-580
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.molcel.2006.01.007
- PMID
- 16483938
- ISSN
- 1097-2765
- eISSN
- 1097-4164
- Language
- English
- Date published
- 2006
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984025252102771
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