The Retinal Ganglion Cell Response to Blast-Mediated Traumatic Brain Injury Is Genetic Background Dependent

Matthew M Harper; Nickolas Boehme; Laura M Dutca; Michael G Anderson

doi:10.1167/iovs.62.7.13

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The Retinal Ganglion Cell Response to Blast-Mediated Traumatic Brain Injury Is Genetic Background Dependent

Matthew M Harper, Nickolas Boehme, Laura M Dutca and Michael G Anderson

Investigative ophthalmology & visual science, Vol.62(7), pp.13-13

06/09/2021

DOI: 10.1167/iovs.62.7.13

PMID: 34106210

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Abstract

Purpose: The purpose of this study was to examine the influence of genetic background on the retinal ganglion cell (RGC) response to blast-mediated traumatic brain injury (TBI) in Jackson Diversity Outbred (J:DO), C57BL/6J and BALB/cByJ mice. Methods: Mice were subject to one blast injury of 137 kPa. RGC structure was analyzed by optical coherence tomography (OCT), function by the pattern electroretinogram (PERG), and histologically using BRN3A antibody staining. Results: Comparison of the change in each group from baseline for OCT and PERG was performed. There was a significant difference in the J:DOΔOCT compared to C57BL/6J mice (P = 0.004), but not compared to BALB/cByJ (P = 0.21). There was a significant difference in the variance of the ΔOCT in J:DO compared to both C57BL/6J and BALB/cByJ mice. The baseline PERG amplitude was 20.33 ± 9.32 µV, which decreased an average of -4.14 ± 12.46 µV following TBI. Baseline RGC complex + RNFL thickness was 70.92 ± 4.52 µm, which decreased an average of -1.43 ± 2.88 µm following blast exposure. There was not a significant difference in the ΔPERG between J:DO and C57BL/6J (P = 0.13), although the variances of the groups were significantly different. Blast exposure in J:DO mice results in a density change of 558.6 ± 440.5 BRN3A-positive RGCs/mm2 (mean ± SD). Conclusions: The changes in retinal outcomes had greater variance in outbred mice than what has been reported, and largely replicated herein, for inbred mice. These results demonstrate that the RGC response to blast injury is highly dependent upon genetic background.

Traumatic Brain Injury

Vision

blast

genetic variation

neurotrauma

pattern electroretinogram

Retina

retinal ganglion cells

Details

Title: Subtitle: The Retinal Ganglion Cell Response to Blast-Mediated Traumatic Brain Injury Is Genetic Background Dependent
Creators: Matthew M Harper - Department of Ophthalmology and Visual Sciences, Carver College of Medicine, The University of Iowa, Iowa City, IA, United States
Nickolas Boehme - Department of Ophthalmology and Visual Sciences, Carver College of Medicine, The University of Iowa, Iowa City, IA, United States
Laura M Dutca - Department of Ophthalmology and Visual Sciences, Carver College of Medicine, The University of Iowa, Iowa City, IA, United States
Michael G Anderson - Department of Ophthalmology and Visual Sciences, Carver College of Medicine, The University of Iowa, Iowa City, IA, United States
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.62(7), pp.13-13
DOI: 10.1167/iovs.62.7.13
PMID: 34106210
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: The Association for Research in Vision and Ophthalmology
Alternative title: Interaction of Blast Injury and Genetics
Language: English
Date published: 06/09/2021
Academic Unit: Molecular Physiology and Biophysics; Biology; Ophthalmology and Visual Sciences
Record Identifier: 9984172168902771

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