The SV40 core sequence functions as a repressor element in yeast

ZHEN-YONG ZHANG-KECK; W. A Kibbe; W. S Moye-Rowley; C. S Parker

doi:10.1016/S0021-9258(18)54645-7

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The SV40 core sequence functions as a repressor element in yeast

Journal article

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Peer reviewed

The SV40 core sequence functions as a repressor element in yeast

ZHEN-YONG ZHANG-KECK, W. A Kibbe, W. S Moye-Rowley and C. S Parker

The Journal of biological chemistry, Vol.266(32), pp.21362-21367

1991

DOI: 10.1016/S0021-9258(18)54645-7

PMID: 1657962

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Abstract

Our previous studies showed that the AP-1 recognition element (ARE) present within the SV40 72-base pair (bp) enhancer will activate transcription in yeast when placed upstream of a truncated CYC1 promoter. However, the AP-2/AP-3 recognition element (also known as the core sequence TGTGGAAAG) from the SV40 enhancer was not able to activate CYC1-dependent transcription. In this report, we show that the core sequence, when cloned next to a yeast UAS (upstream activation sequence), can inhibit the transcriptional stimulatory activity of the UAS. We refer to this sequence as the upstream repressor element (URE) in yeast. Repression occurs in an orientation-independent fashion and irrespective of the placement of the URE between the UAS and TATA box or upstream of both of these elements. Furthermore, repression is seen when the URE is separated from the UAS by up to 214 bp. Interestingly, multiple copies of an activator site can overcome this repression. Gel-shift analysis and URE-probed proteins blots indicate the presence of two polypeptide chains capable of binding the URE in yeast. The experimental evidence suggests that either the repression associated with the URE sequence is mediated by a direct, one-to-one interaction between the proteins recognizing the URE and GCRE, or alternatively, that there is a direct interaction between the activator and repressor for a general transcription factor.

Molecular Genetics

Biological and medical sciences

Fundamental and applied biological sciences. Psychology

Molecular and cellular biology

Transcription. Transcription factor. Splicing. Rna processing

Details

Title: Subtitle: The SV40 core sequence functions as a repressor element in yeast
Creators: ZHEN-YONG ZHANG-KECK - California Institute of Technology
W. A Kibbe - California Institute of Technology
W. S Moye-Rowley - California inst. technology, div. chemistry, Pasadena CA 91125, United States
C. S Parker - California Institute of Technology
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.266(32), pp.21362-21367
DOI: 10.1016/S0021-9258(18)54645-7
PMID: 1657962
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology
Language: English
Date published: 1991
Academic Unit: Molecular Physiology and Biophysics; Internal Medicine
Record Identifier: 9984297611602771

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