The Saccharomyces cerevisiae AP-1 protein discriminates between oxidative stress elicited by the oxidants H2O2 and diamide

John A Wemmie; Susanne M Steggerda; W Scott Moye-Rowley

doi:10.1074/jbc.272.12.7908

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The Saccharomyces cerevisiae AP-1 protein discriminates between oxidative stress elicited by the oxidants H2O2 and diamide

Journal article

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The Saccharomyces cerevisiae AP-1 protein discriminates between oxidative stress elicited by the oxidants H2O2 and diamide

John A Wemmie, Susanne M Steggerda and W Scott Moye-Rowley

The Journal of biological chemistry, Vol.272(12), pp.7908-7914

03/21/1997

DOI: 10.1074/jbc.272.12.7908

PMID: 9065458

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Abstract

The Saccharomyces cerevisiae AP-1 protein (yAP-1) is a key mediator of oxidative stress tolerance. Transcriptional activation by yAP-1 has been shown to be inducible by exposure of cells to H2O2 and diamide, among other oxidative stress eliciting compounds. Here we define the segments of the yAP-1 protein that are required to respond to this environmental challenge. Western blotting analyses indicated that levels of yAP-1 do not change during oxidative stress. Deletion mutagenesis and gene fusion experiments indicate that two different segments of yAP-1 are required for oxidative stress inducibility. These two domains function differentially depending on the type of oxidant used to generate oxidative stress. Three repeated cysteine-serine-glutamate sequences located in the carboxyl terminus are required for normal regulation of yAP-1 function during oxidative stress. Replacement of these cysteine-serine-glutamate repeats by alanine residues does not similarly affect H2O2 and diamide regulation of yAP-1 function. While yAP-1 transactivation is enhanced by exposure to either H2O2 or diamide, the protein responds to the oxidative stress produced by these compounds in nonidentical ways.

Oxidative Stress

Sequence Deletion

Mutagenesis, Site-Directed

Transcription Factor AP-1 - genetics

Bacterial Proteins - genetics

Hydrogen Peroxide - pharmacology

Diamide - pharmacology

Fungal Proteins - genetics

Transcription Factor AP-1 - metabolism

Saccharomyces cerevisiae - metabolism

Cloning, Molecular

Serine Endopeptidases - genetics

Fungal Proteins - metabolism

Details

Title: Subtitle: The Saccharomyces cerevisiae AP-1 protein discriminates between oxidative stress elicited by the oxidants H2O2 and diamide
Creators: John A Wemmie - Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242, USA
Susanne M Steggerda
W Scott Moye-Rowley
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.272(12), pp.7908-7914
DOI: 10.1074/jbc.272.12.7908
PMID: 9065458
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: GM49825 / NIGMS NIH HHS
Language: English
Date published: 03/21/1997
Academic Unit: Molecular Physiology and Biophysics; Psychiatry; Iowa Neuroscience Institute; Neurosurgery; Internal Medicine
Record Identifier: 9984004096902771

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