Journal article
The Salmonella enterica Serovar Typhimurium-Encoded Type III Secretion Systems Can Translocate Chlamydia trachomatis Proteins into the Cytosol of Host Cells
Infection and immunity, Vol.73(2), pp.905-911
02/01/2005
DOI: 10.1128/IAI.73.2.905-911.2005
PMCID: PMC547017
PMID: 15664932
Abstract
Chlamydia trachomatis
is an obligate, intracellular pathogen that is a major cause of preventable blindness and infertility worldwide. Although the published genome sequence suggests that
C. trachomatis
encodes a type III secretion system, the lack of genetic tools for studying
Chlamydia
has hindered the examination of this potentially important class of virulence genes. We have developed a technique to identify
Chlamydia
proteins that can be translocated into the host cell cytoplasm by a type III secretion system. We have selected several
Chlamydia
proteins and tagged them with a multiple peptide motif element called F8M4. Epitopes contained in the F8M4 tag allow us to use tools corresponding to different arms of the adaptive immune system to detect the expression and translocation of these proteins by
Salmonella enterica
serovar Typhimurium. In particular, CD8
+
-T-cell reactivity can be used to detect the translocation of F8M4-tagged proteins into the cytoplasm of host cells. We have found that CD8
+
-T-cell activity assays are sensitive enough to detect translocation of even a small amount of F8M4-tagged protein. We have used CD8
+
-T-cell activity to show that CopN, a
Chlamydia
protein previously shown to be translocated by
Yersinia
type III secretion, can be translocated by the
Salmonella
pathogenicity island 1 (SPI-1) type III secretion system. Additionally, we demonstrate that CopD and Pkn5, two
Chlamydia
proteins hypothesized to be substrates of a type III secretion system, are translocated via the SPI-2 type III secretion system of serovar Typhimurium. The epitope tag system described here can be used more generally to examine the expression and subcellular compartmentalization of bacterial proteins deployed during the interaction of pathogens with mammalian cells.
Details
- Title: Subtitle
- The Salmonella enterica Serovar Typhimurium-Encoded Type III Secretion Systems Can Translocate Chlamydia trachomatis Proteins into the Cytosol of Host Cells
- Creators
- Theresa D. Ho - Harvard UniversityMichael N. Starnbach - Harvard University
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.73(2), pp.905-911
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/IAI.73.2.905-911.2005
- PMID
- 15664932
- PMCID
- PMC547017
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 02/01/2005
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984297328002771
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