Journal article
The Specific Hybridization of p53 Gene on Bead-Quantum Dot Complex in Microfluidic Chip
Journal of nanoscience and nanotechnology, Vol.11(8), pp.7082-7085
08/01/2011
DOI: 10.1166/jnn.2011.4864
PMID: 22103129
Abstract
Recently, nanobiosensors using nanoparticles, such as gold, silver, and quantum dots, have been studied extensively. Among them, fluorescence resonance energy transfer (FRET)-based DNA sensor is prominent device, especially for the medical diagnosis and biomolecular investigations.
FRET is a phenomenon of the emitted energy transfer from one fluorescent dye to another dye through a convoluted wavelength for the excitation. PDMS-based microfluidic chips with pillar structure were prepared for the detection of exon 7 of p53 gene by using QD-DNA probe attached to
polystyrene micro beads. The specific hybridization was investigated with 4 different target oligonucleotides. Fluorescence quenching was observed only from the target oligonucleotide for exon 7 with proper sequence for the hybridization. The fluorescence intensity from QDs decreased rapidly
due to hybridization and FRET between QDs and intercalating dyes.
Details
- Title: Subtitle
- The Specific Hybridization of p53 Gene on Bead-Quantum Dot Complex in Microfluidic Chip
- Creators
- Jeong Ha Yoo - BioengineeringJong Sung Kim
- Resource Type
- Journal article
- Publication Details
- Journal of nanoscience and nanotechnology, Vol.11(8), pp.7082-7085
- Publisher
- American Scientific Publishers
- DOI
- 10.1166/jnn.2011.4864
- PMID
- 22103129
- ISSN
- 1533-4880
- eISSN
- 1533-4899
- Language
- English
- Date published
- 08/01/2011
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9984226818202771
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