Journal article
The Testicular Receptor for Follicle Stimulating Hormone: Structure and Functional Expression of Cloned cDNA
Molecular endocrinology (Baltimore, Md.), Vol.4(4), pp.525-530
04/1990
DOI: 10.1210/mend-4-4-525
PMID: 2126341
Abstract
Cloned cDNA encoding the rat Sertoli cell receptor for FSH was isolated from a cognate library and functionally expressed in cultured mammalian cells. The FSH receptor (FSH-R), as predicted from the cDNA, is a single 75K polypeptide with a 348 residue extracellular domain which contains three N-linked glycosylation sites. This domain is connected to a structure containing seven putative transmembrane segments which displays sequence similarity to G protein-coupled receptors. Thus, the FSH-R is identical in its structural design to the LH/CG receptor (LH/CG-R). Furthermore, both receptors display 50% sequence similarity in their large extracellular domains and 80% identity across the seven transmembrane segments. Expression of the cloned cDNA in mammalian cells conferred FSH-dependent cAMP accumulation. The selectivity for FSH is attested by the fact that the related human glycoprotein hormones human CG and human TSH do not stimulate adenylyl cyclase in FSH-R expressing cells even when these hormones are present at high concentrations.
Details
- Title: Subtitle
- The Testicular Receptor for Follicle Stimulating Hormone: Structure and Functional Expression of Cloned cDNA
- Creators
- Rolf SprengelThomas BraunKaroly NikolicsDeborah L SegaloffPeter H Seeburg
- Resource Type
- Journal article
- Publication Details
- Molecular endocrinology (Baltimore, Md.), Vol.4(4), pp.525-530
- DOI
- 10.1210/mend-4-4-525
- PMID
- 2126341
- ISSN
- 0888-8809
- eISSN
- 1944-9917
- Language
- English
- Date published
- 04/1990
- Academic Unit
- Molecular Physiology and Biophysics; Obstetrics and Gynecology
- Record Identifier
- 9984083862702771
Metrics
9 Record Views