Journal article
The Transmembrane Helix of the Escherichia coli Division Protein FtsI Localizes to the Septal Ring
Journal of bacteriology, Vol.187(1), pp.320-328
01/2005
DOI: 10.1128/JB.187.1.320-328.2005
PMID: 15601716
Abstract
FtsI (also called PBP3) of
Escherichia coli
is a transpeptidase required for synthesis of peptidoglycan in the division septum and is one of about a dozen division proteins that localize to the septal ring. FtsI comprises a short amino-terminal cytoplasmic domain, a single transmembrane helix (TMH), and a large periplasmic domain that encodes the catalytic (transpeptidase) activity. We show here that a 26-amino-acid fragment of FtsI is sufficient to direct green fluorescent protein to the septal ring in cells depleted of wild-type FtsI. This fragment extends from W22 to V47 and corresponds to the TMH. This is a remarkable finding because it is usual for a TMH to target a protein to a site more specific than the membrane. Alanine-scanning mutagenesis of the TMH identified several residues important for septal localization. These residues cluster on one side of an alpha-helix, which we propose interacts directly with another division protein to recruit FtsI to the septal ring.
Details
- Title: Subtitle
- The Transmembrane Helix of the Escherichia coli Division Protein FtsI Localizes to the Septal Ring
- Creators
- Mark C Wissel - Department of Microbiology, University of Iowa, Iowa City, IowaJennifer L Wendt - Department of Microbiology, University of Iowa, Iowa City, IowaCalista J Mitchell - Department of Microbiology, University of Iowa, Iowa City, IowaDavid S Weiss - Department of Microbiology, University of Iowa, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.187(1), pp.320-328
- DOI
- 10.1128/JB.187.1.320-328.2005
- PMID
- 15601716
- NLM abbreviation
- J Bacteriol
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 01/2005
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001130302771
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