Journal article
The Two-Component System ArlRS and Alterations in Metabolism Enable Staphylococcus aureus to Resist Calprotectin-Induced Manganese Starvation
PLoS pathogens, Vol.12(11), pp.e1006040-e1006040
11/01/2016
DOI: 10.1371/journal.ppat.1006040
PMCID: PMC5130280
PMID: 27902777
Abstract
During infection the host imposes manganese and zinc starvation on invading pathogens. Despite this, Staphylococcus aureus and other successful pathogens remain capable of causing devastating disease. However, how these invaders adapt to host-imposed metal starvation and overcome nutritional immunity remains unknown. We report that ArlRS, a global staphylococcal virulence regulator, enhances the ability of S. aureus to grow in the presence of the manganese-and zinc-binding innate immune effector calprotectin. Utilization of calprotectin variants with altered metal binding properties revealed that strains lacking ArlRS are specifically more sensitive to manganese starvation. Loss of ArlRS did not alter the expression of manganese importers or prevent S. aureus from acquiring metals. It did, however, alter staphylococcal metabolism and impair the ability of S. aureus to grow on amino acids. Further studies suggested that relative to consuming glucose, the preferred carbon source of S. aureus, utilizing amino acids reduced the cellular demand for manganese. When forced to use glucose as the sole carbon source S. aureus became more sensitive to calprotectin compared to when amino acids are provided. Infection experiments utilizing wild type and calprotectin-deficient mice, which have defects in manganese sequestration, revealed that ArlRS is important for disease when manganese availability is restricted but not when this essential nutrient is freely available. In total, these results indicate that altering cellular metabolism contributes to the ability of pathogens to resist manganese starvation and that ArlRS enables S. aureus to overcome nutritional immunity by facilitating this adaptation.
Details
- Title: Subtitle
- The Two-Component System ArlRS and Alterations in Metabolism Enable Staphylococcus aureus to Resist Calprotectin-Induced Manganese Starvation
- Creators
- Jana N. Radin - University of Illinois Urbana-ChampaignJessica L. Kelliher - University of Illinois Urbana-ChampaignPaola K. Parraga Solorzano - University of Illinois Urbana-ChampaignThomas E. Kehl-Fie - University of Illinois Urbana-Champaign
- Resource Type
- Journal article
- Publication Details
- PLoS pathogens, Vol.12(11), pp.e1006040-e1006040
- Publisher
- Public Library Science
- DOI
- 10.1371/journal.ppat.1006040
- PMID
- 27902777
- PMCID
- PMC5130280
- ISSN
- 1553-7366
- eISSN
- 1553-7374
- Number of pages
- 22
- Grant note
- R01AI118880 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) K22 AI104805; R01 AI118880 / National Institute of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA 5-FY15-30 / March of Dime Basil O'Conner Starter Scholar Award
- Language
- English
- Date published
- 11/01/2016
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984618508602771
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