The Use of Immunofluorescence in Microdissection Testicular Sperm Extraction

Jason R GREENHALGH; Thomas S GRIFFITH; Moshe WALD

doi:10.2164/jandrol.108.006049

Back

The Use of Immunofluorescence in Microdissection Testicular Sperm Extraction

Journal article

Open access

Peer reviewed

The Use of Immunofluorescence in Microdissection Testicular Sperm Extraction

Jason R GREENHALGH, Thomas S GRIFFITH and Moshe WALD

Journal of andrology, Vol.30(5), pp.548-551

2009

DOI: 10.2164/jandrol.108.006049

PMID: 19234316

Files and links (1)

url

https://doi.org/10.2164/jandrol.108.006049View

Published (Version of record) Open Access

Abstract

Microdissection testicular sperm extraction (TESE) has been used in the treatment of male infertility in cases of nonobstructive azoospermia (NOA). In some NOA cases, there is significant difficulty identifying sperm within the testes, and we propose that an immunofluorescence technique may assist in locating sperm foci for use in in vitro fertilization and intracytoplasmic sperm injection. The purpose of this study was to evaluate the feasibility of this novel technique. Thirteen fertile and 8 sterile mice were anesthetized, and a lower abdominal incision was performed to deliver the testes. Then a small incision was made into the tunica albuginea near the vascular pedicle to expose the seminiferous tubules. A microinjector fitted with a micropipette was used to inject fluorescein isothiocyanate—conjugated mouse anti-human acrosomal IgM antibody (HS-14) into adjacent injection sites along the exposed seminiferous tubules. Following antibody injection, the testes were examined under a Bio-Rad Multiphoton microscope to locate sperm through visualization of fluorescence. Sperm were identified in 22 of 26 testes from fertile mice either by morphologic appearance or by their motility, which was obvious under direct visualization. There was strong binding of the antibody to both the sperm head and tail. In contrast, no sperm were visualized in the sterile mice group. Use of an immunofluorescence technique during microdissection TESE for detection of sperm is a novel and feasible concept. Further studies of this technique are planned.

Fundamental and applied biological sciences. Psychology

Mammalian male genital system

Gynecology. Andrology. Obstetrics

Birth control

Vertebrates: reproduction

Biological and medical sciences

Medical sciences

Sterility. Assisted procreation

Male genital diseases

Details

Title: Subtitle: The Use of Immunofluorescence in Microdissection Testicular Sperm Extraction
Creators: Jason R GREENHALGH - Department of Urology, University of Iowa, Iowa City, Iowa, United States
Thomas S GRIFFITH - Department of Urology, University of Iowa, Iowa City, Iowa, United States
Moshe WALD - Department of Urology, University of Iowa, Iowa City, Iowa, United States
Resource Type: Journal article
Publication Details: Journal of andrology, Vol.30(5), pp.548-551
DOI: 10.2164/jandrol.108.006049
PMID: 19234316
NLM abbreviation: J Androl
ISSN: 0196-3635
eISSN: 1939-4640
Publisher: American Society of Andrology; Schaumburg, IL
Language: English
Date published: 2009
Academic Unit: Urology
Record Identifier: 9984051782402771

Metrics

32 Record Views

11 Times Cited - Web of Science