Journal article
The Utility of Stage-specific Mid-to-late Drosophila Follicle Isolation
Journal of Visualized Experiments, Vol.82, e50493
2013
DOI: 10.3791/50493
PMCID: PMC4011191
PMID: 24326735
Abstract
Drosophila oogenesis or follicle development has been widely used to advance the understanding of complex developmental and cell biologic processes. This methods paper describes how to isolate mid-to-late stage follicles (Stage 10B-14) and utilize them to provide new insights into the molecular and morphologic events occurring during tight windows of developmental time. Isolated follicles can be used for a variety of experimental techniques, including in vitro development assays, live imaging, mRNA expression analysis and western blot analysis of proteins. Follicles at Stage 10B (S10B) or later will complete development in culture; this allows one to combine genetic or pharmacologic perturbations with in vitro development to define the effects of such manipulations on the processes occurring during specific periods of development. Additionally, because these follicles develop in culture, they are ideally suited for live imaging studies, which often reveal new mechanisms that mediate morphological events. Isolated follicles can also be used for molecular analyses. For example, changes in gene expression that result from genetic perturbations can be defined for specific developmental windows. Additionally, protein level, stability, and/or posttranslational modification state during a particular stage of follicle development can be examined through western blot analyses. Thus, stage-specific isolation of Drosophila follicles provides a rich source of information into widely conserved processes of development and morphogenesis.
Details
- Title: Subtitle
- The Utility of Stage-specific Mid-to-late Drosophila Follicle Isolation
- Creators
- Andrew J Spracklen - Department of Anatomy and Cell Biology, University of Iowa Carver College of MedicineTina L Tootle - Department of Anatomy and Cell Biology, University of Iowa Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Journal of Visualized Experiments, Vol.82, e50493
- DOI
- 10.3791/50493
- PMID
- 24326735
- PMCID
- PMC4011191
- NLM abbreviation
- J Vis Exp
- ISSN
- 1940-087X
- eISSN
- 1940-087X
- Publisher
- MyJove Corporation
- Language
- English
- Date published
- 2013
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984025335902771
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