Journal article
The Vps27p Hse1p complex binds ubiquitin and mediates endosomal protein sorting
Nature cell biology, Vol.4(7), pp.534-539
07/2002
DOI: 10.1038/ncb815
PMID: 12055639
Abstract
Membrane proteins that are degraded in the vacuole of Saccharomyces cerevisiae are sorted into discrete intralumenal vesicles, analogous to the internal membranes of multi-vesiculated bodies (MVBs). Recently, it has shown that the attachment of ubiquitin (Ub) mediates sorting into lumenal membranes. We describe a complex of Vps27p and Hse1p that localizes to endosomal compartments and is required for the recycling of Golgi proteins, formation of lumenal membranes and sorting of ubiquitinated proteins into those membranes. The Vps27p-Hse1p complex binds to Ub and requires multiple Ub Interaction Motifs (UIMs). Mutation of these motifs results in specific defects in the sorting of ubiquitinated proteins into the vacuolar lumen. However, the recycling of Golgi proteins and the generation of lumenal membranes proceeds normally in Delta UIM mutants. These data support a model in which the Vps27p-Hse1p complex has multiple functions at the endosome, one of which is as a sorting receptor for ubiquitinated membrane proteins destined for degradation.
Details
- Title: Subtitle
- The Vps27p Hse1p complex binds ubiquitin and mediates endosomal protein sorting
- Creators
- Patricia S Bilodeau - University of IowaJennifer L Urbanowski - University of IowaStanley C Winistorfer - University of IowaRobert C Piper - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Nature cell biology, Vol.4(7), pp.534-539
- DOI
- 10.1038/ncb815
- PMID
- 12055639
- ISSN
- 1465-7392
- eISSN
- 1476-4679
- Grant note
- R01 GM58202 / NIGMS NIH HHS R01 GM058202 / NIGMS NIH HHS
- Language
- English
- Date published
- 07/2002
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297504302771
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