The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia

Alejandro A Pezzulo; Timothy D Starner; Todd E Scheetz; Geri L Traver; Ann E Tilley; Ben-Gary Harvey; Ronald G Crystal; Paul B McCray Jr; Joseph Zabner

doi:10.1152/ajplung.00256.2010

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The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia

Alejandro A Pezzulo, Timothy D Starner, Todd E Scheetz, Geri L Traver, Ann E Tilley, Ben-Gary Harvey, Ronald G Crystal, Paul B McCray Jr and Joseph Zabner

American journal of physiology. Lung cellular and molecular physiology, Vol.300(1), pp.L25-L31

01/2011

DOI: 10.1152/ajplung.00256.2010

PMCID: PMC3023285

PMID: 20971803

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Abstract

Organotypic cultures of primary human airway epithelial cells have been used to investigate the morphology, ion and fluid transport, innate immunity, transcytosis, infection, inflammation, signaling, cilia, and repair functions of this complex tissue. However, we do not know how closely these cultures resemble the airway surface epithelium in vivo. In this study, we examined the genome-wide expression profile of tracheal and bronchial human airway epithelia in vivo and compared it with the expression profile of primary cultures of human airway epithelia grown at the air-liquid interface. For comparison, we also investigated the expression profile of Calu-3 cells grown at the air-liquid interface and primary cultures of human airway epithelia submerged in nutrient media. We found that the transcriptional profile of differentiated primary cultures grown at the air-liquid interface most closely resembles that of in vivo airway epithelia, suggesting that the use of primary cultures and the presence of an air-liquid interface are important to recapitulate airway epithelia biology. We describe a high level of similarity between cells of tracheal and bronchial origin within and between different human donors, which suggests a very robust expression profile that is specific to airway cells.

Trachea - cytology

Humans

Gene Expression Profiling - methods

Cell Culture Techniques - methods

Epithelial Cells - physiology

Culture Media

Tissue Donors - statistics & numerical data

Trachea - physiology

Transcription, Genetic

Bronchi - physiology

Epithelial Cells - cytology

Genome, Human

Bronchi - cytology

Details

Title: Subtitle: The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia
Creators: Alejandro A Pezzulo - Department of Internal Medicine, Univ. of Iowa, Iowa City, 52242, USA
Timothy D Starner
Todd E Scheetz
Geri L Traver
Ann E Tilley
Ben-Gary Harvey
Ronald G Crystal
Paul B McCray Jr
Joseph Zabner
Resource Type: Journal article
Publication Details: American journal of physiology. Lung cellular and molecular physiology, Vol.300(1), pp.L25-L31
DOI: 10.1152/ajplung.00256.2010
PMID: 20971803
PMCID: PMC3023285
NLM abbreviation: Am J Physiol Lung Cell Mol Physiol
ISSN: 1040-0605
eISSN: 1522-1504
Publisher: United States
Grant note: R01-HL-074326 / NHLBI NIH HHS P50-HL-084936 / NHLBI NIH HHS P50-HL-61234 / NHLBI NIH HHS P30 DK054759 / NIDDK NIH HHS UL1-RR-024996 / NCRR NIH HHS N01-AI-30040 / NIAID NIH HHS P30-DK-54759 / NIDDK NIH HHS
Language: English
Date published: 01/2011
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Internal Medicine; Ophthalmology and Visual Sciences
Record Identifier: 9983979970602771

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