Journal article
The brain ryanodine receptor: A caffeine-sensitive calcium release channel
Neuron (Cambridge, Mass.), Vol.7(1), pp.17-25
1991
DOI: 10.1016/0896-6273(91)90070-G
PMID: 1648939
Abstract
The release of stored Ca
2+ from intracellular pools triggers a variety of important neuronal processes. Physiological and pharmacological evidence has indicated the presence of caffeine-sensitive intracellular pools that are distinct from the well-characterized inositol 1,4,5,-trisphosphate (IP
3)-gated pools. Here we report that the brain ryanodine receptor functions as a caffeine- and ryanodine-sensitive Ca
2+ release channel that is distinct from the brain IP
3 receptor. The brain ryanodine receptor has been purified 6700-fold with no change in [
3H]ry-anodine binding affinity and shown to be a homotetramer composed of an approximately 500 kd protein subunit, which is identified by anti-peptide antibodies against the skeletal and cardiac muscle ryanodine receptors. Our results demonstrate that the brain ryanodine receptor functions as a caffeine-sensitive Ca
2+ release channel and thus is the likely gating mechanism for intracellular caffeine-sensitive Ca
2+ pools in neurons.
Details
- Title: Subtitle
- The brain ryanodine receptor: A caffeine-sensitive calcium release channel
- Creators
- Peter S McPhersonx - Program in Neuroscience University of Iowa College of Medicine Iowa City, Iowa 52242 USAYoung-Kee Kim - Howard Hughes Medical Institute University of Iowa College of Medicine Iowa City, Iowa 52242 USAHector Valdivia - Howard Hughes Medical Institute University of Iowa College of Medicine Iowa City, Iowa 52242 USAC.Michael Knudson - Department of Physiology University of Wisconsin Medical School Madison, Wisconsin 53706 USAHiroaki Takekura - Department of Biology and Anatomy University of Pennsylvania Philadelphia, Pennsylvania 19104 USAClara Franzini-Armstrong - Department of Biology and Anatomy University of Pennsylvania Philadelphia, Pennsylvania 19104 USARoberto Coronadot - Howard Hughes Medical Institute University of Iowa College of Medicine Iowa City, Iowa 52242 USAKevin P Campbell - Program in Neuroscience University of Iowa College of Medicine Iowa City, Iowa 52242 USA
- Resource Type
- Journal article
- Publication Details
- Neuron (Cambridge, Mass.), Vol.7(1), pp.17-25
- DOI
- 10.1016/0896-6273(91)90070-G
- PMID
- 1648939
- NLM abbreviation
- Neuron
- ISSN
- 0896-6273
- eISSN
- 1097-4199
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1991
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pathology; Iowa Neuroscience Institute; Radiation Oncology
- Record Identifier
- 9984047853402771
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