The cadherin-11 cytoplasmic juxtamembrane domain promotes alpha-catenin turnover at adherens junctions and intercellular motility

Hans P Kiener; Christopher S Stipp; Philip G Allen; Jonathan M G Higgins; Michael B Brenner

doi:10.1091/mbc.E05-08-0745

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The cadherin-11 cytoplasmic juxtamembrane domain promotes alpha-catenin turnover at adherens junctions and intercellular motility

Journal article

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Peer reviewed

The cadherin-11 cytoplasmic juxtamembrane domain promotes alpha-catenin turnover at adherens junctions and intercellular motility

Hans P Kiener, Christopher S Stipp, Philip G Allen, Jonathan M G Higgins and Michael B Brenner

Molecular biology of the cell, Vol.17(5), pp.2366-2376

05/2006

DOI: 10.1091/mbc.E05-08-0745

PMCID: PMC1446095

PMID: 16525026

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Abstract

Cadherins mediate homophilic cell adhesion and contribute to tissue morphogenesis and architecture. Cadherin cell adhesion contacts are actively remodeled and impact cell movement and migration over other cells. We found that expression of a mutant cadherin-11 lacking the cytoplasmic juxtamembrane domain (JMD) diminished the turnover of alpha-catenin at adherens junctions as measured by fluorescence recovery after photobleaching. This resulted in markedly diminished cell intercalation into monolayers reflecting reduced cadherin-11-dependent cell motility on other cells. Furthermore, the actin cytoskeleton in cadherin-11 deltaJMD cells revealed a more extensive cortical F-actin ring that correlated with significantly higher levels of activated Rac1. Together, these data implicate the cadherin-11 cytoplasmic JMD as a regulator of alpha-catenin turnover at adherens junctions and actin-cytoskeletal organization that is critical for intercellular motility and rearrangement in multicellular clusters.

Mutation

Cytoplasm - chemistry

alpha Catenin - metabolism

Actins - metabolism

Cells, Cultured

Cadherins - analysis

Cytoplasm - metabolism

Cadherins - physiology

Cytoskeleton - ultrastructure

Cell Adhesion

Adherens Junctions - metabolism

Cell Membrane - chemistry

Animals

Cytoskeleton - metabolism

Cell Membrane - metabolism

Mice

Cadherins - genetics

rac1 GTP-Binding Protein - metabolism

Cell Movement

Details

Title: Subtitle: The cadherin-11 cytoplasmic juxtamembrane domain promotes alpha-catenin turnover at adherens junctions and intercellular motility
Creators: Hans P Kiener - Brigham and Women's Hospital
Christopher S Stipp
Philip G Allen
Jonathan M G Higgins
Michael B Brenner - Brigham and Women's Hospital
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.17(5), pp.2366-2376
DOI: 10.1091/mbc.E05-08-0745
PMID: 16525026
PMCID: PMC1446095
NLM abbreviation: Mol Biol Cell
ISSN: 1059-1524
eISSN: 1939-4586
Publisher: American Society for Cell Biology; United States
Grant note: R01 CA086712 / NCI NIH HHS AR48114 / NIAMS NIH HHS R01 AR048114 / NIAMS NIH HHS CA-86712 / NCI NIH HHS
Language: English
Date published: 05/2006
Academic Unit: Molecular Physiology and Biophysics; Biology; Biochemistry and Molecular Biology
Record Identifier: 9983991961802771

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